The Vanderbilt Genome Editing Resource (VGER) provides services, consultation, and collaborations to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt. This resource, which was previously called the Transgenic Mouse ESC Shared Resource (please see announcement letter), has been in existence for over 25 years and has generated 200 unique gene-targeted mice between 1993-2015 and more than 100 genome edited mice using CRISPR-Cas9 since 2014.
We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you.
We provide the following services on a fee-for-service basis:
We no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter).
As always, we are pleased to discuss collaborative projects that involve the development of new strategies and techniques.Contact Us
Danyvid Olivares-Villagómez and his team recently published the first conditional Spp1 mouse model created using CRISPR genome editing through the Vanderbilt Genome Editing Resource. This figure shows Spp1, aka osteopontin, loss of expression specifically in intestinal epithelial cells (IECs) when deletion is driven by IEC-specific Villin-Cre, but not in CD4 T-cells. Altered osteopontin levels are linked with inflammatory bowel disease in humans and clinical trials have explored it as a therapeutic target. This study, published in the Journal of Immunology, demonstrates that osteopontin is critical for the maintenance of intestinal intraepithelial lymphocytes and its loss renders mice susceptible to intestinal inflammation.Read the paper