CRISPR is a rapidly-evolving, multifunctional gene editing tool that enables precise genome modification such as gene deletions, point mutations, addition of various reporters and tags, gene suppression, and gene activation.Find out more today!
In vitro fertilization, rederivation, or ovarian transplantation can be used to introduce new transgenic lines into pathogen-free barrier facilities or recover mice lost due to illness, human error, or aging colonies.Find out more today!
Embryo or sperm cryopreservation protects against the loss of valuable mouse lines from pathogens or disaster. Shipping of gametes is a simplier and more cost-effective way to transfer pathogen-free mice between institutions.Find out more today!
Pronuclear or cytoplasmic microinjections are used to introduce DNA transgenes or CRISPR/Cas9 genome editing reagents (DNA, RNA, or ribonucleoproteins) into mouse zygotes.Find out more today!
Gene-targeted embryonic stem cells are microinjected into blastocysts to generate chimeric founders.
The Vanderbilt Cryopreserved Mouse Repository is a collection of unique strains and genetically-altered alleles that are unavailable at other repositories.
Eric Delpire and his team used CRISPR-Cas9 to create a new mouse model through the TMESCSR carrying an 11-bp deletion in SLC12A2, the gene encoding Na-K-2Cl cotransporter-1. The mutation, originally identified as a heterozygous mutation in a 16 year old patient with multisystem failure, causes a frameshift and early termination of the protein ending in aspartic acid, phenylalanine, STOP (DFX).