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The mission of the Vanderbilt Transgenic Mouse/Embryonic Stem Cell Shared Resource (TMESCSR) is to provide services that facilitate the generation, storage and regeneration of genetically altered mice. We provide a wide array of services that are essential to support these goals. 

The following procedures are provided on a fee-for-service basis:

  • Crispr/Cas9 mouse technologies
  • Gene targeting in mouse ES cells
  • Pronuclear microinjection of DNAs
  • Recombinase Mediated Cassette Exchange
  • Embryo and sperm cryopreservation
  • In vitro fertilization and rederivation
  • BAC recombineering
  • Southern blot hybridization

The TMESCSR is staffed by experienced stem cell scientists and highly competent staff, and our track record in performing these services is extensive.  In addition, we are also happy to work with you to develop and/or implement new methods and techniques.  

  • Location: 9410 MRB-IV
  • Phone: 615-936-3454   

CRISPR mutagenesis: We have now successfully induced knockout mutations in 4 of 5 loci tested to date. The goal of these projects was to create simple insertion/deletion mutations via the NHEJ repair pathway. 

CRISPR editing by homology-directed  gene repair:   We have now had preliminary success in 2 separate codon editing experiments. In each case, 2 or more mice were born carrying a correctly recombined fragment designed to alter a single codon within a targeted gene of interest.

CRISPR/Cas9 Services page + data example


The TMESCSR has now partnered with Sigma-Aldrich to provide discounted core pricing on CRISPR reagents to Vanderbilt customers.

These items include Cas9 mRNA, custom guide RNAs, and long DNA oligos for HDR. Vanderbilt community members may obtain the Sigma-Aldrich CRISPR core price list from this internal web page. 

For more information about Sigma-Aldrich CRISPR reagents, check out the Sigma-Aldrich CRISPR web page or contact Christopher Lemke at Sigma-Aldrich (christopher.lemke@sial.com).   

 

Flexner Discovery Lecture Series: 

Jennifer Doudna, Ph.D.

Crispr Biology:  From Foundational to Translational Science

http://mediasite.vanderbilt.edu/Mediasite/Play/b8701c579ec6438abcc0188275efa16a1d

 

 

Micro-injection

Pronuclear DNA microinjection - Watch video


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