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Bob Coffey and his team designed a CRISPR/Cas9 approach to C-terminally tag the mouse Epithelial Growth Factor Receptor (EGFR) protein with Emerald Green Fluorescent Protein. The TMESCSR introduced the DNA plasmid expressing Cas9 and EGFR-targeted guide RNA along with the modified donor DNA plasmid into mouse zygotes by pronuclear injection.

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