Pronuclear DNA microinjection - View image
The mission of the Vanderbilt Transgenic Mouse/Embryonic Stem Cell Shared Resource (TMESCSR) is to provide services that facilitate the generation, storage and regeneration of genetically altered mice. We provide a wide array of services that are essential to support these goals.
The following procedures are provided on a fee-for-service basis:
The TMESCSR is staffed by experienced stem cell scientists and highly competent staff, and our track record in performing these services is extensive. In addition, we are also happy to work with you to develop and/or implement new methods and techniques.
CRISPR mutagenesis: We have now successfully induced knockout mutations in 4 of 5 loci tested to date. The goal of these projects was to create simple insertion/deletion mutations via the NHEJ repair pathway.
CRISPR editing by homology-directed gene repair: We have now had preliminary success in 2 separate codon editing experiments. In each case, 2 or more mice were born carrying a correctly recombined fragment designed to alter a single codon within a targeted gene of interest.
These items include Cas9 mRNA, custom guide RNAs, and long DNA oligos for HDR. Vanderbilt community members may obtain the Sigma-Aldrich CRISPR core price list from this internal web page.
Pronuclear DNA microinjection - Watch video
|Jennifer Skelton, MS|
|Mark Magnuson, M.D.|
Sr. Scientific Co-Director
|Douglas Mortlock, Ph.D.|
The latest 10 resources contributed to Transgenic Mouse / ESC Shared Resource are available below. (Updated on Mon, 20 Apr 2015)