CRISPR is a rapidly-evolving, multifunctional gene editing tool that enables precise genome modification such as gene deletions, point mutations, addition of various reporters and tags, gene suppression, and gene activation. Edited mouse founders can be obtained in as little as six weeks.Find out more today!
In vitro fertilization, rederivation, or ovarian transplantation can be used to introduce new transgenic lines into pathogen-free barrier facilities or recover mice lost due to illness, human error, or aging colonies.Find out more today!
Embryo or sperm cryopreservation protects against the loss of valuable mouse lines from pathogens or disaster. Shipping of gametes is a simplier and more cost-effective way to transfer pathogen-free mice between institutions.Find out more today!
Pronuclear or cytoplasmic microinjections are used to introduce DNA transgenes or CRISPR/Cas9 genome editing reagents (DNA, RNA, or ribonucleoproteins) into mouse zygotes.Find out more today!
Gene-targeted embryonic stem cells are microinjected into blastocysts to generate chimeric founders.
Bob Coffey and his team designed a CRISPR/Cas9 approach to C-terminally tag the mouse Epithelial Growth Factor Receptor (EGFR) protein with Emerald Green Fluorescent Protein. The TMESCSR introduced the DNA plasmid expressing Cas9 and EGFR-targeted guide RNA along with the modified donor DNA plasmid into mouse zygotes by pronuclear injection.