This plasmid can be used to assemble a gene targeting vector for generating a conditional allele.  It contains two tandemly oriented loxP sites and two tandem FRT sites along with pgk-neo (positive) and pgk-tk (negative) selection cassettes in pBluescript KS+.  The pgk-neo and pgk-tk cassettes were obtained from the plasmid pNTK(A).  Unique restriction enzyme cloning sites are indicated in red. 


Category
Plasmid
Record History
Added on February 9, 2009 at 1:23 PM by Gangula, Rama
Modified on August 18, 2010 at 12:34 PM by Gangula, Rama
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map fh3VLjdP.png
Genbank File pfrt.loxp.gb
Backbone Vector pBluescript KS+
Construct Size 7,012 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided

Source

Laboratory Mark Magnuson
Made byKathy Shelton
Stock Date February 9, 2009

Inventory Location

Private

Attachment

Document frtloxp.pdf - Added on October 2, 2009 at 12:00 AM by Rama Gangula