NOTICE: Labnodes will be unavailable on Sunday March 4th for scheduled maintenance from 6-11AM central time.

The plasmid Alb e/p containing the mouse albumin promoter/enhancer promoter sequence was digested with EcoRV and SAP.  pBS/RIP-Cre-GH was digested with Xba I to retrieve the ~3.3 kb nls-Cre-GH fragment which was subsequently filled-in using Klenow and ligated into the EcoRV site of Alb e/p to create pAlb-Cre-GH/BS.  The final construct was digested with Not I and Kpn I to retrieve the fragment used for microinjection.

Note:  Alb e/p (enhancer/promoter) was originally obtained from Richard Palmiter.

Keywords: transgene liver cre

Category
Plasmid
Record History
Added on December 23, 2008 at 9:08 AM by Gangula, Rama
Modified on September 5, 2013 at 12:54 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map T3jvxIql.png
Genbank File pAlb-Cre-GHBS.gb
Backbone Vector pBluescript KS+
Construct Size 8,626 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided

Source

Laboratory Mark Magnuson
Made byCatherine Postic
Stock Date December 23, 2008

Inventory Location

Private

Publications / Citations

  1. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. Postic C, Shiota M, Niswender KD, Jetton TL, Chen Y, Moates JM, Shelton KD, Lindner J, Cherrington AD, Magnuson MA (1999) J Biol Chem 274(1): 305-15
    › Primary publication · 9867845 (PubMed) · Added on 1/31/2014

MeSH Terms

Albumins Alleles Animals Base Sequence DNA Primers Gene Deletion Glucokinase Glucose Homeostasis Insulin Integrases Islets of Langerhans Liver Mice Mice, Knockout Transgenes Viral Proteins