Common Name | Ptf1aflox |
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VCMR ID | TC |
Date Cryopreserved | 2014-09-16 |
Method of Cryopreservation | Sperm |
Trial IVF % Fertilization | 84.48% |
Mutation #1: Targeted Mutagenesis | |||
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Allele |
Name: pancreas specific transcription factor, 1a; targeted mutation 3, Christopher VE Wright Symbol: Ptf1atm3Cvw MGI: MGI:5788429 |
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Zygosity at cryopreservation | Homozygote | ||
PCR Genotyping Protocol | Ptf1aflox_Genotyping_Protocol.docx | ||
Citations |
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Strain Type | Mixed |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Cryopreservation Strain Background (VCMR) | C57BL/6J |
Viability and Fertility Data | Strain Background: 50% C57BL/6J at cryopreservation Normal number of pups; No breeding issues; Homozygous viable. |
5' loxP site was placed 1.6kb upstream of the transcription start site. The FRT-Neo-FRT cassette was placed 100 bp after the transcription stop site followed by the 3' loxP. Upon germline transmission, the neomycin cassette was deleted by a FLPe deleter mouse.