|Method of Cryopreservation||Sperm|
|Trial IVF % Fertilization||84.48%|
|Mutation #1: Targeted Mutagenesis|
Name: pancreas specific transcription factor, 1a; targeted mutation 3, Christopher VE Wright
|Zygosity at cryopreservation||Homozygote|
|PCR Genotyping Protocol||Ptf1aflox_Genotyping_Protocol.docx|
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Cryopreservation Strain Background (VCMR)||C57BL/6J|
|Viability and Fertility Data||
Strain Background: 50% C57BL/6J at cryopreservation
Normal number of pups; No breeding issues; Homozygous viable.
5' loxP site was placed 1.6kb upstream of the transcription start site. The FRT-Neo-FRT cassette was placed 100 bp after the transcription stop site followed by the 3' loxP. Upon germline transmission, the neomycin cassette was deleted by a FLPe deleter mouse.