Conditional null allele of Ptf1a.LoxP sites flanking exon 1 and 2 (entire coding region of Ptf1a).


Record History
Added on February 12, 2014 at 1:43 PM by Peters, Jody
Modified on December 6, 2018 at 9:31 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name Ptf1aflox
VCMR ID TC
Date Cryopreserved 2014-09-16
Method of Cryopreservation Sperm
Trial IVF % Fertilization 84.48%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: pancreas specific transcription factor, 1a; targeted mutation 3, Christopher VE Wright
Symbol: Ptf1atm3Cvw
MGI: MGI:5788429
Zygosity at cryopreservation Homozygote
PCR Genotyping Protocol Ptf1aflox_Genotyping_Protocol.docx
Citations
Publication
The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma. (2015) Elife 4: (Added 8/4/2015)
PMID: 26151762

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Strain Background:  50% C57BL/6J at cryopreservation

Normal number of pups; No breeding issues; Homozygous viable.

Additional Information

5' loxP site was placed 1.6kb upstream of the transcription start site.  The FRT-Neo-FRT cassette was placed 100 bp after the transcription stop site followed by the 3' loxP. Upon germline transmission, the neomycin cassette was deleted by a FLPe deleter mouse.