Conditional null allele of Ptf1a.LoxP sites flanking exon 1 and 2 (entire coding region of Ptf1a).


Record History
Added on February 12, 2014 at 1:43 PM by Peters, Jody
Modified on December 6, 2018 at 9:31 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Ptf1aflox
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID TC
Additional Strain Information

5' loxP site was placed 1.6kb upstream of the transcription start site.  The FRT-Neo-FRT cassette was placed 100 bp after the transcription stop site followed by the 3' loxP. Upon germline transmission, the neomycin cassette was deleted by a FLPe deleter mouse.  

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleConditional Null
Targeted GeneName: Pancreas Specific Transcription Factor, 1a
Symbol:Ptf1a
Allele Name: pancreas specific transcription factor, 1a; targeted mutation 3, Christopher VE Wright
Symbol: Ptf1atm3Cvw
MGI: MGI:5788429
Description of Targeting Vector

5' loxP site was placed 1.6kb upstream of the transcription start site.  The FRT-Neo-FRT cassette was placed 100 bp after the transcription stop site followed by the 3' loxP. Upon germline transmission, the neomycin cassette was deleted by a FLPe deleter mouse.  


Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol Ptf1aflox_Genotyping_Protocol.docx
Citations
Publication
The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma. (2015) Elife 4: (Added 8/4/2015)
PMID: 26151762

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 1
Strain Description

50% C57Bl6/J at cryopreservation

Cryopreserved in 2014.

Trial IVF 84.48% fertilization rate.