This mouse contains a cyan fluorescent protein (CFP, Cerulean), tagged with 3 copies of an SV40 NLS knocked into the endogenous Pdx1 gene.  This mouse strain enables prepancreatic endoderm to be easily identified in the developing mouse embryo and the isolation of Pdx1(+) cells by FACS.

 


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on July 7, 2017 at 12:48 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name Pdx1CFP
Research Applications Fluorescent proteins
MMRRC ID 036462-UNC
Jackson Laboratories Stock No Not provided
VUCMR ID KX, LE
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleGene Replacement
Targeted GeneName: pancreatic and duodenal homeobox 1
Symbol: Pdx1
NCBI: 18609
Allele Name: targeted mutation 1.2
Symbol: Pdx1tm1.2(CFP)
MGI: 5438568
Description of Targeting Vector

Coding sequences for a nuclear localized Cerulean followed by the beta globin splice/polyA sequence were inserted into exon 1 of the Pdx1 locus.  Using recombinase-mediated cassette exchange (RMCE), the cassette was inserted into the Pdx1LCA. The exchange cassette also contains a flrted (flanked by FRT) pgk-Hygro sequence for positive selection after RMCE.  The pgk-Hygro cassette is removed after germline transmission.


Vector Genbank File pPdx1.TV.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Type of Allele
Exchanged Cassette Gene Name (18609)
Exchanged Cassette Allele Name Pdx1{LCA}
Description of Exchange Vector

None Provided

Exchanged Cassette Genbank File Pdx1.CFP.BpA_.gb
Citations
Publication
A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1. (2012) Genesis 50: 384-92 (Added 12/5/2013)
PMID: 21913313

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background CD-1
Number of Generations Backcrossed 9
Strain Description

Mice containing the Pdx1CFP allele were bred to FlpE-expressing transgenic mice to remove the hygroR selection cassette.  Animals with this allele are currently being maintained in a CD1 (outbred) genetic background. 

Publications / Citations

  1. A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1. Potter LA, Choi E, Hipkens SB, Wright CV, Magnuson MA (2012) Genesis 50(4): 384-92
    › Primary publication · 21913313 (PubMed) · PMC3870909 (PubMed Central) · Added on 12/5/2013

MeSH Terms

Alleles Animals Cell Differentiation Embryonic Stem Cells Endoderm Female Flow Cytometry Gene Expression Regulation, Developmental Gene Regulatory Networks Genes, Reporter Gene Targeting Green Fluorescent Proteins Homeodomain Proteins Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Fluorescence Mutagenesis, Insertional Pancreas Recombinases Trans-Activators

Attachments

Document pdx1_cfp_for_bcbc.png - Added on July 19, 2010 at 10:17 AM by Mark Magnuson


Document pdx1.cfp_hygro_pcr_protocol.doc - Added on July 29, 2010 at 1:31 PM by Jill Lindner

PCR protocol for genotyping mice