These mice were generated using RMCE to insert an exchange vector containing a modified Rosa26 promoter linked to a Cerulean fluorescent protein (CFP) reporter gene into mESCs containing a Loxed Cassette Acceptor (LCA) allele within the Rosa26 gene locus.  The Rosa26 promoter in this mouse was altered by replacing DNA sequences from 228 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box.  This mouse will facilitate studies of retinoic acid signaling in an intact animal. 


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on October 31, 2019 at 2:27 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository
Request this Mouse Line

Mouse Information

Common Name Rosa26R26-228-DR5-TA-Cerulean
Date Cryopreserved 2017-08-28
Method of Cryopreservation Sperm
Trial IVF % Fertilization 27.72%

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Allele Name: targeted mutation 1.2
Symbol: Gt(ROSA)26Sortm1.2(R26-228-DR5-Cerulean)Mgn
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol R2660_and_228_genotyping_protocol.docx
Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. (2012) Dis Model Mech 5: 956-66 (Added 11/6/2013)
PMID: 22888097

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Strain Background:  93.75% C57Bl6/J at cryopreservation.

Heterozygous viable and fertile.

Additional Information

A Rosa26 RMCE plasmid constructed for use with the ES cell loci targeted by pRosa26.LCA. In this experiment native Rosa26 gene sequence from -228 to +81 was replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean red reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested.


Document r26228dr5tacerh_pcr_protocol.doc - Added on July 27, 2010 at 2:11 PM by Jill Lindner

PCR protocol for genotyping mice

Document R26-228-DR5-TA-Cerulean.png - Added on July 19, 2010 at 10:17 AM by Mark Magnuson