These mice were generated using RMCE to insert an exchange vector containing a modified Rosa26 promoter linked to a Cerulean fluorescent protein (CFP) reporter gene into mESCs containing a Loxed Cassette Acceptor (LCA) allele within the Rosa26 gene locus.  The Rosa26 promoter in this mouse was altered by replacing DNA sequences from 228 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box.  This mouse will facilitate studies of retinoic acid signaling in an intact animal. 

 


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on December 6, 2018 at 9:32 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Rosa26R26-228-DR5-TA-Cerulean
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID KW
Additional Strain Information

A Rosa26 RMCE plasmid constructed for use with the ES cell loci targeted by pRosa26.LCA. In this experiment native Rosa26 gene sequence from -228 to +81 was replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean red reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested.

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleGene Replacement
Targeted GeneName: gene trap ROSA 26, Philippe Soriano
Symbol: Gt(ROSA)26Sor
NCBI: 14910
Allele Name: targeted mutation 1.2
Symbol: Gt(ROSA)26Sortm1.2(R26-228-DR5-Cerulean)Mgn
Description of Targeting Vector

A Rosa26 RMCE plasmid constructed for use with the ES cell loci targeted by pRosa26.LCA. In this experiment native Rosa26 gene sequence from -228 to +81 was replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean red reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested.


Vector Genbank File pRosa26.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol R2660_and_228_genotyping_protocol.docx
Type of Allele Gene Replacement
Exchanged Cassette Gene Name tumor necrosis factor receptor superfamily, member 10b (DR5)
Exchanged Cassette Allele Name Rosa26{LCA}
Description of Exchange Vector

Not Provided

Exchanged Cassette Genbank File R26-228-DR5-TA-Cerulean.gb
PCR Genotyping Protocol Not provided
Citations
Publication
Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. (2012) Dis Model Mech 5: 956-66 (Added 11/6/2013)
PMID: 22888097

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 2 generations backcrossed to C57BL6/J
Strain Description

129S6 germline chimeras were backcrossed for two generations to C57BL/6J.

93.75% C57Bl6/J at cryopreservation.

Cryopreserved in 2010.

IVF 2017 32.38% fertilization rate.

Attachments

Document r26228dr5tacerh_pcr_protocol.doc - Added on July 27, 2010 at 2:11 PM by Jill Lindner

PCR protocol for genotyping mice


Document R26-228-DR5-TA-Cerulean.png - Added on July 19, 2010 at 10:17 AM by Mark Magnuson