Common Name | Rosa26R26-60-DR5-TA-Cerulean |
---|---|
VCMR ID | KV |
Date Cryopreserved | 2010-03-16 |
Method of Cryopreservation | Sperm |
Trial IVF % Fertilization | 6.00% |
Mutation #1: RMCE Targeted Mutagenesis | |||
---|---|---|---|
Allele |
Name: targeted mutation 1.1 Symbol: Gt(ROSA)26Sortm1.1(R26-60-DR5-Cerulean)Mgn |
||
Zygosity at cryopreservation | Heterozygote | ||
PCR Genotyping Protocol | R2660_and_228_genotyping_protocol.docx | ||
Citations |
|
Strain Type | Congenic Strain |
---|---|
Chimera/Founder Genetic Background | 129S6/SvEvTac |
Cryopreservation Strain Background (VCMR) | C57BL/6J |
Viability and Fertility Data | Strain Background: 96.97% C57Bl6/J at cryopreservation. Heterozygous viable and fertile. |
Through homologous recombination in ES cells, a 5.165 kb region of the Rosa26 gene containing exon 1 was replaced by a floxed tk-neo cassette, a puromycin-delta-TK fusion gene driven by the mouse phosphoglycerol kinase promoter (puroR-delta-TK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites (Cre-recombinase recognition sequences).
r2660dr5tacerh_pcr_protocol.doc - Added on July 27, 2010 at 1:55 PM by Jill Lindner
PCR protocol for genotyping mice
R26-60-DR5-TA-Cerulean.png - Added on July 19, 2010 at 10:17 AM by Mark Magnuson
Rosa26-60-DR5-TA-Cerulean gene targeting and RMCE strategy.