This is a retinoic acid responsive CFP reporter allele. In this allele, the Rosa26 promoter was modified using RMCE, replacing DNA sequences from -60 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box. 


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on December 6, 2018 at 9:31 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Rosa26R26-60-DR5-TA-Cerulean
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID KV
Additional Strain Information

Through homologous recombination in ES cells, a 5.165 kb region of the Rosa26 gene containing exon 1 was replaced by a floxed tk-neo cassette, a puromycin-delta-TK fusion gene driven by the mouse phosphoglycerol kinase promoter (puroR-delta-TK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites (Cre-recombinase recognition sequences).

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleGene Replacement
Targeted GeneName: gene trap ROSA 26, Philippe Soriano
Symbol: Gt(ROSA)26Sor
NCBI: 14910
Allele Name: targeted mutation 1.1
Symbol: Gt(ROSA)26Sortm1.1(R26-60-DR5-Cerulean)Mgn
Description of Targeting Vector

Through homologous recombination in ES cells, a 5.165 kb region of the Rosa26 gene containing exon 1 was replaced by a floxed tk-neo cassette, a puromycin-delta-TK fusion gene driven by the mouse phosphoglycerol kinase promoter (puroR-delta-TK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites (Cre-recombinase recognition sequences).


Vector Genbank File pRosa26.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol R2660_and_228_genotyping_protocol.docx
Type of Allele Gene Replacement
Exchanged Cassette Gene Name (CFP)
Exchanged Cassette Allele Name Rosa26{tm1.1(R26-DR5-TA-Cerulean)}
Description of Exchange Vector

In the R26-60-DR5-TA-Cerulean exchange vector a native Rosa26 gene sequence from -60 to +81 is replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean(CFP) fluorescent reporter. The vector also contains RMCE-enabling Lox66/Lox2272 sites  and Pgk-hygromycin resistance cassette flanked by tandem FRT sites, enabling excision of the cassette after germline transmission.

Exchanged Cassette Genbank File R26-60-DR5-TA-Cerulean.gb
PCR Genotyping Protocol Not provided
Citations
Publication
Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. (2012) Dis Model Mech 5: 956-66 (Added 11/6/2013)
PMID: 22888097

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 2
Strain Description

Germline 129S6 chimeras were backcrossed to C57BL6/J for two generations.

96.97% C57Bl6/J at cryopreservation.

Cryopreserved in 2010.

Attachments

Document r2660dr5tacerh_pcr_protocol.doc - Added on July 27, 2010 at 1:55 PM by Jill Lindner

PCR protocol for genotyping mice


Document R26-60-DR5-TA-Cerulean.png - Added on July 19, 2010 at 10:17 AM by Mark Magnuson

Rosa26-60-DR5-TA-Cerulean gene targeting and RMCE strategy.