Insm1{GFPCre} mice contain a GFP-Cre fusion protein which replaces the Insm1 coding sequence. These mice express green fluorescent protein (GFP) under control of the Insm1 gene locus. Insm1 is expressed in pancreatic primordium starting at E9.5. Insm1 is also expressed in neural precursor cells and tumors of may be used for lineage tracing of Insm1-positive cells in both wild-type and Insm1-null mice.


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on April 6, 2017 at 5:06 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name Insm1GFP.Cre
Research Applications Cre-lox system
Fluorescent proteins
MMRRC ID 36986-Jax
Jackson Laboratories Stock No 023899
VUCMR ID JX
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleGene Replacement
Targeted GeneName: insulinoma-associated 1
Symbol: Insm1
NCBI: 53626
Allele Name: targeted mutation 1.1
Symbol: Insm1tm1.1(GFP-Cre)
Description of Targeting Vector

Through recombinase-mediated cassette exchange in ES cells, a 5232 bp fragment containing a GFP-Cre fusion gene was inserted into the Insm1 locus replacing the entire coding sequence of Insm1 gene. To facilitate positive selection of cassette-exchanged ES cells, a Pgk-Hygro selection cassette flanked by FRT sites was placed immediate downstream of the GFP-Cre fusion gene.


Vector Genbank File pGT-Insm1.L71-2272_Gb.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Type of Allele
Exchanged Cassette Gene Name (53626)
Exchanged Cassette Allele Name Insm1{LCA}
Description of Exchange Vector

Not Provided

Exchanged Cassette Genbank File Insm1GFP.Cre.gb
Citations Not provided

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background CD-1
Number of Generations Backcrossed 10
Strain Description

Founder chimeras were bred with CD-1 mice for more than 10 generations.

Attachments

Document insm1.gfpcre.hygro_or__protocol._ks.doc - Added on July 27, 2010 at 12:35 PM by Jill Lindner

PCR protocol for genotyping mice


Document Insm1.GFP.Cre.png - Added on July 19, 2010 at 10:17 AM by Mark Magnuson

Insm1.GFP gene targeting strategy