Common Name | Insm1GFP.Cre |
---|---|
Research Applications |
Cre-lox system Fluorescent proteins |
MMRRC ID | 36986-Jax |
Jackson Laboratories Stock No | 023899 |
VCMR ID | JX |
Additional Strain Information | Not provided |
Mutation #1: RMCE Targeted Mutagenesis | |
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Type of Allele | Gene Replacement |
Targeted Gene | Name: insulinoma-associated 1 Symbol: Insm1 NCBI: 53626 |
Allele |
Name: targeted mutation 1.1 Symbol: Insm1tm1.1(GFP-Cre) |
Description of Targeting Vector | Through recombinase-mediated cassette exchange in ES cells, a 5232 bp fragment containing a GFP-Cre fusion gene was inserted into the Insm1 locus replacing the entire coding sequence of Insm1 gene. To facilitate positive selection of cassette-exchanged ES cells, a Pgk-Hygro selection cassette flanked by FRT sites was placed immediate downstream of the GFP-Cre fusion gene. |
Vector Genbank File |
pGT-Insm1.L71-2272_Gb.gb |
Allele Map | Not Provided |
PCR Genotyping Protocol | Not provided |
Type of Allele | |
Exchanged Cassette Gene Name | (53626)
|
Exchanged Cassette Allele Name | Insm1{LCA} |
Description of Exchange Vector | Not Provided |
Exchanged Cassette Genbank File |
Insm1GFP.Cre.gb |
PCR Genotyping Protocol |
Not provided |
Citations | Not provided |
Strain Type | Inbred Strain |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Current Genetic Background | CD-1 |
Number of Generations Backcrossed | 10 |
Strain Description | Founder chimeras were bred with CD-1 mice for more than 10 generations. |
insm1.gfpcre.hygro_or__protocol._ks.doc - Added on July 27, 2010 at 12:35 PM by Jill Lindner
PCR protocol for genotyping mice
Insm1.GFP.Cre.png - Added on July 19, 2010 at 10:17 AM by Mark Magnuson
Insm1.GFP gene targeting strategy