Ptf1a{YFP} mice may be used to visualize yellow flurrescent protein (YFP) expression from the ptf1a allele.  Ptf1a is expressed in pancreatic buds beginning at E9.5 and plays a vital role in the growth and lineage specification of pancreatic multipotent progenitor cells (MPCs).  Ptf1a is also expressed in the neuronal precursors of the cerebellum, spinal cord, and retina where it also performs fate determining roles. 

 


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on April 14, 2017 at 1:27 PM by Skelton, Jennifer
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name ptf1aYFP
Research Applications FLP-FRT system
Fluorescent proteins
MMRRC ID 029175-MU/H
Jackson Laboratories Stock No Not provided
VUCMR ID GZ, HA
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleGene Replacement
Targeted GeneName: pancreas specific transcription factor, 1a
Symbol: Ptf1a
NCBI: 1328312
Allele Name: targeted mutation 1.1
Symbol: ptf1atm1.1(YFP)
MGI: 3691484
Description of Targeting Vector

Coding sequences for citrine, a yellow fluorescent protein, were inserted into exon 1 of the Ptf1a locus. Briefly, the endogenous NotI site in exon 1 of Ptf1a was mutated to a FseI site. Citrine DNA was PCR amplified with a 5' Kozak consensus sequence and cloned in between the unique FseI and MluI sites within exon 1 of Ptf1a which replaces sequences containing 5' UTR as well as a portion of the Ptf1a coding region.


Vector Genbank File Ptf1a.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Type of Allele
Exchanged Cassette Gene Name (19213)
Exchanged Cassette Allele Name ptf1a{LCA}
Description of Exchange Vector

Not Provided

Exchanged Cassette Genbank File Ptf1a_LFP_KO_(-hygro).gb
Citations
Publication
Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. (2008) Dev Biol 316: 74-86 (Added 9/3/2013)
PMID: 18294628

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background CD-1
Number of Generations Backcrossed 8
Strain Description

After achieving germline transmission, mice carrying the ptf1aYFP+hygroR allele were mated to a FlpE-expressing transgenic line in order to remove the FRT flanked hygroR cassette.  Ptf1aYFP mice were then backcrossed to CD-1 outbred mice for eight generationss.

Attachments

Document ptf1a.yfph__w_pcr_protocol.doc - Added on July 27, 2010 at 1:02 PM by Jill Lindner

PCR protocol for genotyping mice


Document Ptf1a.YFP_-_hygro.png - Added on July 19, 2010 at 10:17 AM by Mark Magnuson

Ptf1a.YFP gene targeting strategy