|Jackson Laboratories Stock No||Not provided|
|Additional Strain Information||Not provided|
|Mutation #1: Targeted Mutagenesis|
|Type of Allele||Conditional Null|
|Targeted Gene||Name: Peroxisome proliferator-activated receptor gamma|
Name: targeted mutation 1.1Mgn
|Description of Targeting Vector||
A gene targeting strategy that utilizes Cre/loxP was used to generate mice that contain loxP sites flanking exon 2 of the Pparg gene, thus generating a conditional allele. Genotype by DNA PCR using primers 5'-GCT CCT GAG TGC TAA TAT TAA AG-3' and 5'-CCA TGG ACT AAT GCT GTA ATA TTA-3' primers. These primers amplify a 572 bp loxed allele and a 464 bp wild type allele. Homozygous animals are viable and do not exhibit any obvious mutant phenotype. Heterozygous mice are viable and do not exhibit any obvious mutant phenotype.
|Vector Genbank File||
|Allele Map||Not Provided|
|PCR Genotyping Protocol||Not provided|
|Strain Type||Congenic Strain|
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Current Genetic Background||C57BL/6J|
|Number of Generations Backcrossed||12|
Mice carrying the Pparglox allele were backcrossed to C57BL/6J for 12 generations.