These mice carry a conditional allele for PPARgamma (γ) that can be used in combination with various cre-expressing transgenes.

 


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on April 13, 2017 at 3:11 PM by Skelton, Jennifer
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name PPARgammalox
Research Applications Cre-lox system
MMRRC ID 012035-MU/H
Jackson Laboratories Stock No Not provided
VUCMR ID CG
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleConditional Null
Targeted GeneName: Peroxisome proliferator-activated receptor gamma
Symbol: Pparg
NCBI: 19016
Allele Name: targeted mutation 1.1Mgn
Symbol: Ppargtm1.1Mgn
MGI: 2385456
Description of Targeting Vector

A gene targeting strategy that utilizes Cre/loxP was used to generate mice that contain loxP sites flanking exon 2 of the Pparg gene, thus generating a conditional allele. Genotype by DNA PCR using primers 5'-GCT CCT GAG TGC TAA TAT TAA AG-3' and 5'-CCA TGG ACT AAT GCT GTA ATA TTA-3' primers. These primers amplify a 572 bp loxed allele and a 464 bp wild type allele. Homozygous animals are viable and do not exhibit any obvious mutant phenotype. Heterozygous mice are viable and do not exhibit any obvious mutant phenotype.


Vector Genbank File mPPARG.KO.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Citations
Publication
Generation and functional confirmation of a conditional null PPARgamma allele in mice. (2002) Genesis 32: 134-7 (Added 8/13/2010)
PMID: 11857800

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 12
Strain Description

Mice carrying the Pparglox allele were backcrossed to C57BL/6J for 12 generations.

Attachments

Document Pparg_Jones_et_al_2002_Page_2_Image_0001.jpg - Added on July 19, 2010 at 10:17 AM by Mark Magnuson

PPARgamma gene targeting strategy


Document pparglox__wt_pcr_protocol.doc - Added on July 27, 2010 at 12:51 PM by Jill Lindner

PCR protocol for genotyping mice.