Common Name | rictorlox |
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Research Applications |
Cre-lox system LacZ |
MMRRC ID | 014113-UNC |
Jackson Laboratories Stock No | Not provided |
VCMR ID | Not provided |
Additional Strain Information | Not provided |
Mutation #1: Targeted Mutagenesis | |||
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Type of Allele | Conditional Null | ||
Targeted Gene | Name: Rictor Symbol: 4921505C17Rik NCBI: 78757 | ||
Allele |
Name: targeted mutation 1.1 Symbol: 4921505C17Riktm1.1Mgn MGI: MGI:3703321 |
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Description of Targeting Vector | A conditional allele was generated using a 3 loxP plus 2 FRT site strategy in which 2 FRT sites flank partial exon 3, which is linked to the lacZ reporter plus a neoR cassette. 2 loxP sites flank the neoR cassette and the third loxP flanks exon 3 of the gene. Mice containing the RiklacZ+neotarget were subsequently bred to Flpe transgenics. The resulting mice contain intact exon3 flanked by loxP sites plus one remaining FRT site. Genotype by DNA PCR utilizing 5'-ACTGAATATGTTCATGGTTGTG-3' and 5'-GAAGTTATTCAGATGGCCCAGC-3'. These primers amplify a 554 bp targeted allele and a 466 bp wild type allele. |
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Vector Genbank File |
pGEM-Pia-Target.gb | ||
Allele Map | Not Provided | ||
PCR Genotyping Protocol | Not provided | ||
Citations |
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Strain Type | Insipient congenic |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Current Genetic Background | C57BL/6J |
Number of Generations Backcrossed | 5 |
Strain Description | Mice containing the RiklacZ+neo allele were bred to Flpe-expressing transgenic mice. The offspring contain an intact exon 3 flanked by tandem loxP sites and a residual FRT site. This allele is also called riclox or ricEx3cond. |
pia.lox__wt_pcr_protocol.doc - Added on July 27, 2010 at 1:27 PM by Jill Lindner
PCR protocol for genotyping mice