Rictor is an essential part of mTOR complex 2 (mTORC2).  mTORC2 phosphorylates Ser473 of Akt/PKB.  Eliminating this gene disrupts the function of mTORC2, thereby preventing growth factor mediated activation of Akt/PKB.  Thus, these mice have utility for studying both the sites of expression and function of  rictor.  

 


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on October 5, 2015 at 3:13 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name RictorlacZ
Research Applications LacZ
MMRRC ID 015200-UCD
Jackson Laboratories Stock No Not provided
VUCMR ID Not provided
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleGlobal Null
Targeted GeneName: Rictor
Symbol: 4921505C17Rik
NCBI: 78757
Allele Name: targeted mutation 1.3
Symbol: 4921505C17Riktm2Mgn
MGI: 3703325
Description of Targeting Vector

This knock-in (null) allele was made using a multi-allelic gene targeting strategy that involved the use of both FRT and loxP sites. After germline transmission a neoR cassette and exon 3 (which were flanked by loxP sites) were removed by mating to EIIa-Cre transgenic mice, thereby generating mice containing the riclacZ allele. This riclacZ allele contains a lacZ-pA region flanked on the 5' side by a single (remnant) FRT site and on the 3' side by a single loxP site. Genotype by DNA PCR utilizing primers 5'-ATT GCA GCT TAT AAT GGT TAC AA-3' and 5'-GAC ACT GGA TTA CAG TGG CTT G-3'. These primers amplify a 295 bp riclacZ allele while primers 5'-ACT GAA TAT GTT CAT GGT TGT G-3' and 5'-GAA GTT ATT CAG ATG GCC CAG C-3' amplify a 466 bp ric wild type allele. Homozygous animals exhibit an embryonic lethal phenotype. Embryos exhibit growth arrest after E9.5 and die by E11.5. Heterozygous animals are viable and do not exhibit any obvious mutant phenotype.


Vector Genbank File pGEM-Pia-Target.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Citations
Publication
Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability. (2006) Dev Cell 11: 583-9 (Added 1/12/2012)
PMID: 16962829

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 6
Strain Description

A multiallelic gene targeting strategy was used to generate a riclacZ+neo allele in mouse embryonic stem cells.  After germline transmission both the neoR cassette and exon 3 were removed by mating to EIIa-Cre transgenic mice, thereby generating mice that express the riclacZ allele.  RiclacZ mice were subsequently bacakcrossed into a C57BL6/J background.  This is a null allele.

Attachments

Document pia.lacz__wt_pcr_protocol.doc - Added on July 27, 2010 at 2:51 PM by Jill Lindner

PCR protocol for genotyping mice


Document Rictor_image.jpg - Added on July 19, 2010 at 10:17 AM by Mark Magnuson