These mice may be used to study tissue-specific functions of phosphoenolpyruvate carboxykinase  (Pck1).  This enzyme is essential for gluconeogenesis and also is important for regulating anapleurosis/catapleurosis of TCA cycle intermediates.  By generating mice that are homozygous for the pck{lox} allele and that contain a tissue-specific Cre transgene Pck1 can be deleted from various sites.

 

Keywords: Pck1 pcklox PEPCK

Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on April 6, 2017 at 4:52 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name pck1lox
Research Applications Cre-lox system
MMRRC ID 011950-UNC
Jackson Laboratories Stock No Not provided
VUCMR ID AV, AX
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleConditional Null
Targeted GeneName: phosphoenolpyruvate carboxykinase 1
Symbol: Pck1
NCBI: 18534
Allele Name: targeted mutation 1.1, Mark A Magnuson
Symbol: Pck1tm1.1Mgn
MGI: 2449283
Description of Targeting Vector

A gene targeting strategy was used to flank exons 4 and 5 in the Pck1 gene with two tandomly-oriented loxP sites. DNA PCR utilizing primers 5'-AATGTTCTCTGCAAGTCCTGGTG-3' and 5'-TCTGTCAGTTCAATACCAATCT-3' amplify a 616 bp pckloxband and a 518 bp wild type band. Homozygous Pcklox/lox animals are viable. Pck1 activity and protein content in liver and kidney do not differ from wild type. Heterozygous animals are also viable and do not differ from the wild type.


Vector Genbank File pmPEPCK.KO2.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Citations
Publication
Hepatic energy state is regulated by glucagon receptor signaling in mice. (2009) J Clin Invest 119: 2412-22 (Added 3/18/2013)
PMID: 19662685
Cytosolic phosphoenolpyruvate carboxykinase does not solely control the rate of hepatic gluconeogenesis in the intact mouse liver. (2007) Cell Metab 5: 313-20 (Added 4/10/2012)
PMID: 17403375
Impaired tricarboxylic acid cycle activity in mouse livers lacking cytosolic phosphoenolpyruvate carboxykinase. (2004) J Biol Chem 279: 48941-9 (Added 4/10/2012)
PMID: 15347677
Mechanisms by which liver-specific PEPCK knockout mice preserve euglycemia during starvation. (2003) Diabetes 52: 1649-54 (Added 12/10/2013)
PMID: 12829628
Phosphoenolpyruvate carboxykinase is necessary for the integration of hepatic energy metabolism. (2000) Mol Cell Biol 20: 6508-17 (Added 12/10/2013)
PMID: 10938127

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background 99.76% C57BL/6
Number of Generations Backcrossed 10
Strain Description

Mice carrying the pcklox allele have been backcrossed ten times into a C57Bl/6J background.

Attachments

Document mb170054901a.jpeg - Added on July 19, 2010 at 10:17 AM by Mark Magnuson


Document pcklox__pckwt_pcr_protocol.doc - Added on July 27, 2010 at 10:56 AM by Jill Lindner

PCR protocol for genotyping mice