Common Name | pck1lox |
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Research Applications |
Cre-lox system |
MMRRC ID | 011950-UNC |
Jackson Laboratories Stock No | Not provided |
VCMR ID | AV, AX |
Additional Strain Information | Not provided |
Mutation #1: Targeted Mutagenesis | |||||||
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Type of Allele | Conditional Null | ||||||
Targeted Gene | Name: phosphoenolpyruvate carboxykinase 1 Symbol: Pck1 NCBI: 18534 | ||||||
Allele |
Name: targeted mutation 1.1, Mark A Magnuson Symbol: Pck1tm1.1Mgn MGI: MGI:2449283 |
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Description of Targeting Vector | A gene targeting strategy was used to flank exons 4 and 5 in the Pck1 gene with two tandomly-oriented loxP sites. DNA PCR utilizing primers 5'-AATGTTCTCTGCAAGTCCTGGTG-3' and 5'-TCTGTCAGTTCAATACCAATCT-3' amplify a 616 bp pckloxband and a 518 bp wild type band. Homozygous Pcklox/lox animals are viable. Pck1 activity and protein content in liver and kidney do not differ from wild type. Heterozygous animals are also viable and do not differ from the wild type. |
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Vector Genbank File |
pmPEPCK.KO2.gb | ||||||
Allele Map | Not Provided | ||||||
PCR Genotyping Protocol | Not provided | ||||||
Citations |
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Strain Type | Congenic Strain |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Current Genetic Background | 99.76% C57BL/6 |
Number of Generations Backcrossed | 10 |
Strain Description | Mice carrying the pcklox allele have been backcrossed ten times into a C57Bl/6J background. |
pcklox__pckwt_pcr_protocol.doc - Added on July 27, 2010 at 10:56 AM by Jill Lindner
PCR protocol for genotyping mice