Gk{lox} mice may be used to generate cell specific knock-outs of glucokinase, depending which cre-expressing transgenic mouse is used.  In humans, glucokinase gene mutations cause maturity onset diabetes of the young (MODY-GK), a disease that is characterized by early onset and persistent hyperglycemia. Thus, these mice are useful in determining how diminished experssion of glucokinase in specific cells causes hyperglycemia.

 

 


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on October 5, 2015 at 1:25 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name gklox
Research Applications Cre-lox system
MMRRC ID 011949-UNC
Jackson Laboratories Stock No Not provided
VUCMR ID Not provided
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleConditional Null
Targeted GeneName: Glucokinase
Symbol: Gck
NCBI: 103988
Allele Name: targeted mutation 1.1
Symbol: Gcktm1.1Mgn
MGI: 2177709
Description of Targeting Vector

A gene targeting strategy was used to flank exons 9 and 10 in the glucokinase gene with two tandomly-oriented loxP sites. This strain allows for the tissue specific knock-out of glucokinase to be made. For example, crossing the gklox/lox mice with an insulin-cre transgenic mouse generates a beta cell specific knock-out of glucokinase. Genotype by DNA PCR using primers 5'-TGT CTC AAT TTG CTG TGT CCT CCA-3' and 5'-TCT GTT AAT GCA AAT GCT CGT GTT-3'. A 710 bp band will be amplified for the gklox allele and a 605 bp band for the wild type allele. Homozygous gklox/lox mice are viable but have a blood glucose concentrations slightly higher than wild types (194 +/- 3 mg/dl vs. 175 +/- 8 mg/dl). This finding suggests that the insertion of a loxP site (and some flanking sequences) between exons 8 and 9 may have caused a slight attenuation in glucokinase gene expression compared to mice with two wild type alleles.


Vector Genbank File pBOB.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Citations
Publication
Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. (1999) J Biol Chem 274: 305-15 (Added 1/31/2014)
PMID: 9867845

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 10
Strain Description

After achieving germline transmission mice carrying the gklox allele were backcrossed for ten generations into a C57Bl/6J background.

Attachments

Document gk_g__w__pcr_protocol.doc - Added on July 27, 2010 at 9:52 AM by Jill Lindner

PCR protocol for genotyping mice.


Document GK_lox_target.jpg - Added on July 19, 2010 at 10:17 AM by Mark Magnuson

GK lox targeting