These mice, which contain an inactivating, K414E mutation within the Gck gene, provide a model for Maturity Onset Diabetes of the Young, or MODY-GK.

During the generation of this line, male chimeric mice derived by injecting correctly targeted TL1 mESCs into blastocysts from C57Bl/6 animals were mated directly with 129S6 female mice, thereby maintaining the mutation in an inbred state. 

Cryostocks of both 8-cell embryos and sperm from this line exist only at the VCMR.


Record History
Added on July 19, 2010 at 10:17 AM by Magnuson, Mark
Modified on September 4, 2019 at 1:33 PM by Skelton, Jennifer
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Public: Shared Collection
VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name gkK414E
VCMR ID GP, GQ
Date Cryopreserved 2007-01-08
Method of Cryopreservation Embryo

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: glucokinase; targeted mutation 2, Mark A Magnuson
Symbol: Gcktm2Mgn
MGI: MGI:3701697
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol Gck.K414_PCR_genotyping_protocol.docx
Citations
Publication
Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations. (2007) J Biol Chem 282: 13906-16 (Added 12/10/2013)
PMID: 17353190

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) 129S6/SvEvTac
Viability and Fertility Data

Strain background:  100% 129S6 at cryopreservation

Vial ID GP:  embryos at the 8-cell stage.

Vial ID GQ:  sperm cryopreserved in vials.

Homozygous lethal at P2.

Heterozygous viable and fertile.

Neo cassette not removed for 129S6 strain.

Attachments

Document gkk414e.neo__wt_protocol.doc - Added on July 27, 2010 at 10:28 AM by Jill Lindner

PCR protocol for genotyping mice.


Document K414EA456V_targeting_vector.jpg - Added on July 19, 2010 at 10:17 AM by Mark Magnuson

K414E and A456V targeting vectors