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|Research Applications||Not provided|
|MMRRC ID||Not provided|
|Jackson Laboratories Stock No||Not provided|
|VCMR ID||GP, GQ|
|Additional Strain Information||Not provided|
|Mutation #1: Targeted Mutagenesis|
|Type of Allele||Global Mutation|
|Targeted Gene||Name: Glucokinase|
Name: glucokinase; targeted mutation 2, Mark A Magnuson
|Description of Targeting Vector||
A single base pair mutation was introduced into exon 9 via site specific mutagenesis to change amino acid 414 from lysine to glutamic acid. Genotype by DNA PCR using primers 5'-TGT CTC AAT TTG CTG TGT CCT CCA-3' and 5'-ATG TGT GAG TGT GCC AAT ATG AGT-3'. These primers will amplify a 636 bp fragment from the wild type allele and a 741 bp fragment from the targeted (mutant) allele. These animals are homozygous lethal. Heterozygous mice are viable. They are hyperglycemic and hypoinsulinemic when compared to wild type.
|Vector Genbank File||
|Allele Map||Not Provided|
|PCR Genotyping Protocol||Gck.K414_PCR_genotyping_protocol.docx|
|Strain Type||Inbred Strain|
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Current Genetic Background||Not provided|
|Number of Generations Backcrossed||NA|
100% 129S6 at cryopreservation
Cryopreserved in 2007.
Vial ID GP - embryos at the 8 cell stage.
Vial ID GQ - sperm cryopreserved in vials.