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Common Name | gkK414E |
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Research Applications | Not provided |
MMRRC ID | Not provided |
Jackson Laboratories Stock No | Not provided |
VCMR ID | GP, GQ |
Additional Strain Information | Not provided |
Mutation #1: Targeted Mutagenesis | |||
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Type of Allele | Global Mutation | ||
Targeted Gene | Name: Glucokinase Symbol: Gck NCBI: 103988 | ||
Allele |
Name: glucokinase; targeted mutation 2, Mark A Magnuson Symbol: Gcktm2Mgn MGI: MGI:3701697 |
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Description of Targeting Vector | A single base pair mutation was introduced into exon 9 via site specific mutagenesis to change amino acid 414 from lysine to glutamic acid. Genotype by DNA PCR using primers 5'-TGT CTC AAT TTG CTG TGT CCT CCA-3' and 5'-ATG TGT GAG TGT GCC AAT ATG AGT-3'. These primers will amplify a 636 bp fragment from the wild type allele and a 741 bp fragment from the targeted (mutant) allele. These animals are homozygous lethal. Heterozygous mice are viable. They are hyperglycemic and hypoinsulinemic when compared to wild type. |
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Vector Genbank File |
pBOB2.K414E.gb | ||
Allele Map | Not Provided | ||
PCR Genotyping Protocol | Gck.K414_PCR_genotyping_protocol.docx | ||
Citations |
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Strain Type | Inbred Strain |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Current Genetic Background | Not provided |
Number of Generations Backcrossed | NA |
Strain Description | 100% 129S6 at cryopreservation Cryopreserved in 2007. Vial ID GP - embryos at the 8 cell stage. Vial ID GQ - sperm cryopreserved in vials. |
gkk414e.neo__wt_protocol.doc - Added on July 27, 2010 at 10:28 AM by Jill Lindner
PCR protocol for genotyping mice.
K414EA456V_targeting_vector.jpg - Added on July 19, 2010 at 10:17 AM by Mark Magnuson
K414E and A456V targeting vectors