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Common Name | gkA456V |
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VCMR ID | DE, DS |
Date Cryopreserved | 2004-11-08 |
Method of Cryopreservation | Embryo |
Mutation #1: Targeted Mutagenesis | |||
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Allele |
Name: glucokinase; targeted mutation 3, Mark A Magnuson Symbol: Gcktm3Mgn MGI: MGI:3701764 |
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Zygosity at cryopreservation | Heterozygote | ||
PCR Genotyping Protocol | Gck.A456V_PCR_genotyping_protocol.docx | ||
Citations |
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Strain Type | Congenic Strain |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Cryopreservation Strain Background (VCMR) | 129S6/SvEvTac |
Viability and Fertility Data | Strain background: Backcrossed >10 generations onto 129S6/SvEvTac. Vial DE: cryopreserved embryos at the 8-cell stage Vial DS: cryopreserved sperm in vials Homozygous viable. Heterozygous viable and fertile. Neo cassette was not removed for 129S6 strain. |
gka456v.neo__wt_protocol.doc - Added on July 27, 2010 at 10:38 AM by Jill Lindner
PCR protocol for genotyping mice
K414EA456V_targeting_vector.jpg - Added on July 19, 2010 at 10:16 AM by Mark Magnuson
K414E and A456V targeting vectors