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|Research Applications||Not provided|
|MMRRC ID||Not provided|
|Jackson Laboratories Stock No||Not provided|
|VCMR ID||DE, DS|
|Additional Strain Information||Not provided|
|Mutation #1: Targeted Mutagenesis|
|Type of Allele||Global Mutation|
|Targeted Gene||Name: Glucokinase|
Name: glucokinase; targeted mutation 3, Mark A Magnuson
|Description of Targeting Vector||
A single base mutation was introduced into exon 10 via site specific mutagenesis to change amino acid 456 from alanine to valine. Genotype by DNA PCR using primers 5'-TGT CTC AAT TTG CTG TGT CCT CCA-3' and 5'-ATG TGT GAG TGT GCC AAT ATG AGT-3'. These primers will amplify a 636 bp fragment from the wild type allele and a 741 bp fragment from the mutant allele. Homozygous mutant mice, which have a phenotype of moderate hypoglycemia, are viable and breed well. Heterozygous animals are mildly hypoglycemic.
|Vector Genbank File||
|Allele Map||Not Provided|
|PCR Genotyping Protocol||Gck.A456V_PCR_genotyping_protocol.docx|
|Strain Type||Congenic Strain|
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Current Genetic Background||Not provided|
|Number of Generations Backcrossed||>10|
Need to check how neo cassette was removed.
129SvEv at cryopreservation
Cryopreserved in 2005.
Vial DE - cryopreserved embryos at the 8 cell stage
Vial DS - cryopreserved sperm in vials