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Common Name | gkA456V |
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Research Applications | Not provided |
MMRRC ID | Not provided |
Jackson Laboratories Stock No | Not provided |
VCMR ID | DE, DS |
Additional Strain Information | Not provided |
Mutation #1: Targeted Mutagenesis | |||
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Type of Allele | Global Mutation | ||
Targeted Gene | Name: Glucokinase Symbol: Gck NCBI: 103988 | ||
Allele |
Name: glucokinase; targeted mutation 3, Mark A Magnuson Symbol: Gcktm3Mgn MGI: MGI:3701764 |
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Description of Targeting Vector | A single base mutation was introduced into exon 10 via site specific mutagenesis to change amino acid 456 from alanine to valine. Genotype by DNA PCR using primers 5'-TGT CTC AAT TTG CTG TGT CCT CCA-3' and 5'-ATG TGT GAG TGT GCC AAT ATG AGT-3'. These primers will amplify a 636 bp fragment from the wild type allele and a 741 bp fragment from the mutant allele. Homozygous mutant mice, which have a phenotype of moderate hypoglycemia, are viable and breed well. Heterozygous animals are mildly hypoglycemic. |
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Vector Genbank File |
pBOB2.A456V.gb | ||
Allele Map | Not Provided | ||
PCR Genotyping Protocol | Gck.A456V_PCR_genotyping_protocol.docx | ||
Citations |
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Strain Type | Congenic Strain |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Current Genetic Background | Not provided |
Number of Generations Backcrossed | >10 |
Strain Description | Need to check how neo cassette was removed. 129SvEv at cryopreservation Cryopreserved in 2005. Vial DE - cryopreserved embryos at the 8 cell stage Vial DS - cryopreserved sperm in vials |
gka456v.neo__wt_protocol.doc - Added on July 27, 2010 at 10:38 AM by Jill Lindner
PCR protocol for genotyping mice
K414EA456V_targeting_vector.jpg - Added on July 19, 2010 at 10:16 AM by Mark Magnuson
K414E and A456V targeting vectors