This plasmid is used for expressing the liver/hepatic isoform of glucokinase in eukaryotic cell lines.  It contains a rat glucokinase cDNA (hepatic isoform) fragment that was generated by PCR amplification, then cloned into pCMV4, a plasmid that drives expression from using a cytomegalovirus promoter, a translational augmenter from alfalfa mosaic virus and 3’ splice and polyadenylation signals from human growth hormone gene.   


Record History
Added on December 19, 2008 at 6:13 PM by Magnuson, Mark
Modified on September 5, 2013 at 12:47 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map ybeZljP5.png
Genbank File
Backbone Vector pCMV4
Construct Size 6,279 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided


Laboratory Mark Magnuson
Made byTod Martin
Stock Date December 19, 2008

Inventory Location


Publications / Citations

  1. Effects of alternate RNA splicing on glucokinase isoform activities in the pancreatic islet, liver, and pituitary. Liang Y, Jetton TL, Zimmerman EC, Najafi H, Matschinsky FM, Magnuson MA (1991) J Biol Chem 266(11): 6999-7007
    › Primary publication · 2016311 (PubMed) · Added on 1/16/2014

MeSH Terms

Animals Base Sequence Cell Line Exons Glucokinase Hexokinase Islets of Langerhans Isoenzymes Liver Mice Molecular Sequence Data Mutagenesis, Insertional Mutagenesis, Site-Directed Oligonucleotide Probes Pituitary Gland Polymerase Chain Reaction Rats Restriction Mapping RNA, Messenger RNA Splicing Sequence Homology, Nucleic Acid Transfection