A transgenic construct containing a 2.34 kb rat albumin enhancer/promoter, nuclear localization sequence-modified Cre recombinase and a 2.1 kb fragment from the human growth hormone gene was injected into B6D2(F2) pronuclei.  This strain lacks any overt phenotype related to the transgene.  It has been well characterized and shown to be very efficient for performing liver-specific gene knockouts using Cre/loxP system.  This line has been shown to be nearly 100% efficient in achieving liver-specific recombination when crossed with multiple different floxed alleles. 

 


Record History
Added on October 14, 2013 at 10:37 AM by Peters, Jody
Modified on April 14, 2017 at 1:18 PM by Skelton, Jennifer
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name Alb-Cre
Research Applications Cre-lox system
MMRRC ID Not provided
Jackson Laboratories Stock No 003574
VUCMR ID KN, P
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: BAC or Transgene Insertion
Type of Vector Plasmid
Promoter Name: Albumin
Symbol: Alb
Expressed Gene Name: Cre recombinase
Symbol: Cre
NCBI Gene ID: 2777477
Description of Transgene Not provided
Vector Genbank File pAlbCreGHBS.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 8+
Strain Description

99.9% C57Bl6/J

Publications / Citations

  1. DNA excision in liver by an albumin-Cre transgene occurs progressively with age. Postic C, Magnuson MA (2000) Genesis 26(2): 149-50
    › Primary publication · 10686614 (PubMed) · Added on 1/31/2014
  2. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. Postic C, Shiota M, Niswender KD, Jetton TL, Chen Y, Moates JM, Shelton KD, Lindner J, Cherrington AD, Magnuson MA (1999) J Biol Chem 274(1): 305-15
    › Primary publication · 9867845 (PubMed) · Added on 1/31/2014

MeSH Terms

Aging Albumins Alleles Animals Base Sequence DNA DNA Primers Gene Deletion Glucokinase Glucose Homeostasis Insulin Integrases Islets of Langerhans Liver Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Recombination, Genetic Transgenes Viral Proteins

Attachments

Document AlbCre_Location_Specific_PCR_Protocol.doc - Added on January 30, 2014 at 12:24 PM by Jody Peters

Location Specific PCR genotyping protocol


Document AlbCre_PCR_Protocol.doc - Added on January 30, 2014 at 11:16 AM by Jody Peters

Transgene-specific PCR genotyping protocol


Document pAlbCreGH_BS.doc - Added on January 31, 2014 at 9:59 AM by Jody Peters

Map and DNA sequence of plasmid from which the transgene was isolated.