|Start Date / Time||October 1, 2014 at 9:00 AM|
|End Date / Time||October 1, 2014 at 9:55 AM|
|Location||512 Light Hall|
|Presenter Name||Nicholas Vierra (Jacobson)|
|Presentation Title||β-cell TALK-1: illuminating the function of an islet-specific K+ channel|
|Status||This meeting has already occurred|
TALK-1 is a two-pore domain K+ channel expressed specifically in the islets of Langerhans. Although a nonsynonymous CDS polymorphism in TALK-1 has been shown to contribute to type 2 diabetes susceptibility, nothing is known about the role of this channel in β-cells. We show that genetic ablation of TALK-1 alters islet Ca2+ dynamics and glucose-stimulated insulin secretion (GSIS). Furthermore, we have identified that TALK-1 is sensitive to membrane phospholipids, which increase channel activity through a charge-dependent interaction with the C-terminus of TALK-1. These data provide compelling evidence that TALK-1 modulates GSIS by limiting excitability, and suggests a novel mechanism for phospholipid-dependent regulation of islet Ca2+ entry.