Current and ongoing beta cell research is presented in this weekly seminar by faculty, postdoctoral fellows and students. If you are interested in attending the Beta Cell Interest Group (BIG) seminars and joining the BIG community, please contact David Jacobson.

Keywords: beta cell BIG

Record History
Added on September 11, 2013 at 11:47 AM by Brown, Deborah
Modified on May 27, 2014 at 3:16 PM by Brown, Deborah
Shared with (contributions)
BIG: Beta Cell Interest Group (BIG) Seminar Master

Meeting Details

Start Date / Time June 4, 2014 at 9:00 AM
End Date / Time June 4, 2014 at 9:55 AM
Duration 55 minutes
Location 512 Light Hall
Presenter Name Brian McKenna (Stein)
Presentation Title The identification and characterization of Pdx1 coregulators
Status This meeting has already occurred

Meeting Agenda/Notes

Multiple lines of evidence implicate the transcription factor Pancreatic Duodenal Homeobox-1 (Pdx1) as being a master regulator of pancreas development and mature b-cell function.  Like many other transcription factors, Pdx1 relies on its ability to recruit coregulators to mediate its transcriptional action.  However, to date candidate approaches have only identified HDAC1/2, Set7/9, PCIF, and p300 as Pdx1 coregulators.  Therefore, using the Reversible Cross-Link ImmunoPrecipitation (RE-CLIP) technique coupled with mass spectrometry, we unbiasedly isolated a striking number of novel Pdx1-interacting factors.  Among the confirmed interacting factors were multiple members of the ATP-dependent chromatin-remodeling complex, Swi/Snf, a well-established transcription modulator of developmental and mature cell processes.  My presentation will be focused on our analysis on the two essential and mutually exclusive core enzymatic Swi/Snf ATPase subunits, Brg1 and Brm, and their contribution to Pdx1 transcriptional action in b-cells.