|Start Date / Time||June 4, 2014 at 9:00 AM|
|End Date / Time||June 4, 2014 at 9:55 AM|
|Location||512 Light Hall|
|Presenter Name||Brian McKenna (Stein)|
|Presentation Title||The identification and characterization of Pdx1 coregulators|
|Status||This meeting has already occurred|
Multiple lines of evidence implicate the transcription factor Pancreatic Duodenal Homeobox-1 (Pdx1) as being a master regulator of pancreas development and mature b-cell function. Like many other transcription factors, Pdx1 relies on its ability to recruit coregulators to mediate its transcriptional action. However, to date candidate approaches have only identified HDAC1/2, Set7/9, PCIF, and p300 as Pdx1 coregulators. Therefore, using the Reversible Cross-Link ImmunoPrecipitation (RE-CLIP) technique coupled with mass spectrometry, we unbiasedly isolated a striking number of novel Pdx1-interacting factors. Among the confirmed interacting factors were multiple members of the ATP-dependent chromatin-remodeling complex, Swi/Snf, a well-established transcription modulator of developmental and mature cell processes. My presentation will be focused on our analysis on the two essential and mutually exclusive core enzymatic Swi/Snf ATPase subunits, Brg1 and Brm, and their contribution to Pdx1 transcriptional action in b-cells.