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Ca Ski

This is an example of a human cell line for teaching purposes.
 
The line was established from cells from a metastasis in the small bowel mesentery.
Record History
Added on August 14, 2013 at 11:12 AM by Lindner, Jill
Modified on August 14, 2013 at 11:27 AM by Lindner, Jill
Shared with (contributions)
VCSCBi: Training / Cell Lines Master

Cell Line Annotations

Organism Human (Homo sapiens)
Tissue Cervix
Growth Properties adherent
Morphology epithelial
Antibiotics Not provided
Trypsin 0.25% (w/v) Trypsin - 0.53 mM EDTA
Passage Number Not provided
Splitting Ratio 1:3 to 1:8
Media Change Every 2 to 3 days
Media Recipe ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001 + 10$ FBS
Mycoplasma Not contaminated
Biosafety Level 2

Source

Commercial ATCC
Catalog #ATCC® CRL-1550™
Stock Date August 14, 2013

Inventory Location

Private

Publications / Citations

  1. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Hendricks DT, Taylor R, Reed M, Birrer MJ (1997) Cancer Res 57(11): 2112-5
    › Primary publication · 9187105 (PubMed) · Added on 8/14/2013
  2. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. Baker CC, Phelps WC, Lindgren V, Braun MJ, Gonda MA, Howley PM (1987) J Virol 61(4): 962-71
    › Primary publication · 3029430 (PubMed) · PMC254051 (PubMed Central) · Added on 8/14/2013
  3. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Pater MM, Pater A (1985) Virology 145(2): 313-8
    › Primary publication · 2992153 (PubMed) · Added on 8/14/2013
  4. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Yee C, Krishnan-Hewlett I, Baker CC, Schlegel R, Howley PM (1985) Am J Pathol 119(3): 361-6
    › Primary publication · 2990217 (PubMed) · PMC1888002 (PubMed Central) · Added on 8/14/2013
  5. Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Pattillo RA, Hussa RO, Story MT, Ruckert AC, Shalaby MR, Mattingly RF (1977) Science 196(4297): 1456-8
    › Primary publication · 867042 (PubMed) · Added on 8/14/2013

MeSH Terms

Acid Anhydride Hydrolases Adult Amino Acid Sequence Antigens, Neoplasm Base Sequence Blotting, Southern Cell Line Cell Migration Inhibition Cells, Cultured Cell Transformation, Viral Chorionic Gonadotropin Cloning, Molecular Culture Media DNA, Neoplasm DNA, Viral DNA Restriction Enzymes Endometrial Neoplasms Female Gene Expression Regulation, Neoplastic Genes Genes, Viral Humans Leukocytes Neoplasm Proteins Nucleic Acid Heteroduplexes Nucleic Acid Hybridization Ovarian Neoplasms Papillomaviridae Polymerase Chain Reaction Protein Biosynthesis Proteins RNA, Messenger RNA, Viral Sequence Analysis, DNA Transcription, Genetic Tumor Cells, Cultured Uterine Cervical Neoplasms

Attachments

Document 028278BEF34C49799451515BBC2FE79E.pdf - Added on August 14, 2013 at 11:12 AM by Jill Lindner

Document CRL1550.pdf - Added on August 14, 2013 at 11:10 AM by Jill Lindner

Document msds_animal.pdf - Added on August 14, 2013 at 11:11 AM by Jill Lindner