This is an example of a mouse cell line for teaching purposes.
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.

Record History
Added on August 14, 2013 at 10:59 AM by Lindner, Jill
Modified on February 13, 2015 at 10:51 AM by Lindner, Jill
Shared with (contributions)
VCSCBi: Training / Cell Lines Master

Cell Line Annotations

Organism Mouse (Mus musculus)
Tissue Breast
Growth Properties adherent
Morphology epithelial
Antibiotics Not provided
Trypsin 0.25% trypsin-0.53mM EDTA
Passage Number Not provided
Splitting Ratio 1:6 to 1:8
Media Change Every 2 to 3 days
Media Recipe ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001 + 10% FBS
Mycoplasma Not contaminated
Biosafety Level 1


Commercial ATCC
Catalog #ATCC® CRL-2539™
Stock Date August 14, 2013

Inventory Location


Publications / Citations

  1. Cooperativity of Staphylococcal aureus enterotoxin B superantigen, major histocompatibility complex class II, and CD80 for immunotherapy of advanced spontaneous metastases in a clinically relevant postoperative mouse breast cancer model. Pulaski BA, Terman DS, Khan S, Muller E, Ostrand-Rosenberg S (2000) Cancer Res 60(10): 2710-5
    › Primary publication · 10825145 (PubMed) · Added on 8/14/2013
  2. Immunotherapy with vaccines combining MHC class II/CD80+ tumor cells with interleukin-12 reduces established metastatic disease and stimulates immune effectors and monokine induced by interferon gamma. Pulaski BA, Clements VK, Pipeling MR, Ostrand-Rosenberg S (2000) Cancer Immunol Immunother 49(1): 34-45
    › Primary publication · 10782864 (PubMed) · Added on 8/14/2013
  3. Reduction of established spontaneous mammary carcinoma metastases following immunotherapy with major histocompatibility complex class II and B7.1 cell-based tumor vaccines. Pulaski BA, Ostrand-Rosenberg S (1998) Cancer Res 58(7): 1486-93
    › Primary publication · 9537252 (PubMed) · Added on 8/14/2013
  4. Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor. Aslakson CJ, Miller FR (1992) Cancer Res 52(6): 1399-405
    › Primary publication · 1540948 (PubMed) · Added on 8/14/2013

MeSH Terms

Animals Antigens, Neoplasm Antimetabolites, Antineoplastic B7-1 Antigen Cancer Vaccines CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Cell Division Cytotoxicity, Immunologic Disease Models, Animal Drug Resistance, Neoplasm Enterotoxins Female Histocompatibility Antigens Class II Humans Immunotherapy Immunotherapy, Active Interferon-gamma Interleukin-12 Liver Neoplasms Lung Neoplasms Lymphatic Metastasis Mammary Neoplasms, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Nude Neoplasm Metastasis Neoplasm Transplantation Postoperative Care Staphylococcus aureus Superantigens T-Lymphocytes T-Lymphocytes, Cytotoxic Thioguanine Transfection Tumor Stem Cell Assay Vaccination


Document CRL2539.pdf - Added on August 14, 2013 at 10:59 AM by Jill Lindner

Document msds_animal.pdf - Added on August 14, 2013 at 10:59 AM by Jill Lindner