This is an example of a hamster cell line for teaching purposes.
 
The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.

Record History
Added on August 14, 2013 at 10:33 AM by Lindner, Jill
Modified on November 6, 2014 at 3:41 PM by Lindner, Jill
Shared with (contributions)
VCSCBi: Training / Cell Lines Master

Cell Line Annotations

Organism Hamster (Cricetulus griseus)
Tissue Ovary
Growth Properties adherent
Morphology epithelial-like
Antibiotics Not provided
Trypsin 0.25% (w/v) Trypsin- 0.53 mM EDTA
Passage Number Not provided
Splitting Ratio 1:4 to 1:8
Media Change Every 2 to 3 days
Media Recipe ATCC-formulated F-12K Medium, Catalog No. 30-2004 + 10%FBS
Mycoplasma Not contaminated
Biosafety Level 1

Source

Commercial ATCC
Catalog #ATCC® CCL-61™
Stock Date August 14, 2013

Inventory Location

Private

Publications / Citations

  1. CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM. HAM RG (1965) Proc Natl Acad Sci U S A : 288-93
    › Primary publication · 14294058 (PubMed) · PMC219509 (PubMed Central) · Added on 9/24/2013
  2. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. PUCK TT, CIECIURA SJ, ROBINSON A (1958) J Exp Med 108(6): 945-56
    › Primary publication · 13598821 (PubMed) · PMC2136918 (PubMed Central) · Added on 8/14/2013
  3. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Kao FT, Puck TT (1968) Proc Natl Acad Sci U S A 60(4): 1275-81
    › Primary publication · 5244736 (PubMed) · PMC224914 (PubMed Central) · Added on 8/14/2013

MeSH Terms

Animals Bromodeoxyuridine Cell Culture Techniques Cell Division Chromosomes Clone Cells Cricetinae Culture Media Culture Techniques Female Humans L Cells (Cell Line) Mammals Metabolism Mice Mutagens Nitroso Compounds Ovary Research Tissue Culture Techniques

Attachments

Document CCL61.pdf - Added on August 14, 2013 at 10:35 AM by Jill Lindner


Document CHO_K1.png - Added on August 14, 2013 at 10:30 AM by Jill Lindner


Document msds_animal.pdf - Added on August 14, 2013 at 10:36 AM by Jill Lindner