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This plasmid will express the islet isoform of glucokinase in eukaryotic cell lines.  It contains a rat glucokinase cDNA (islet isoform) cloned into pCMV4, a plasmid that drives expression from using a cytomegalovirus promoter, a translational augmenter from alfalfa mosaic virus and 3’ splice and polyadenylation signals from human growth hormone gene.   


Category
Plasmid
Record History
Added on December 19, 2008 at 6:01 PM by Magnuson, Mark
Modified on September 5, 2013 at 12:46 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map uhSbVi6L.png
Genbank File pCMV4.GKB1.gb
Backbone Vector pCMV4
Construct Size 7,080 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided

Source

Laboratory Mark Magnuson
Made byKathy Shelton
Stock Date December 19, 2008

Inventory Location

Private

Publications / Citations

  1. Effects of alternate RNA splicing on glucokinase isoform activities in the pancreatic islet, liver, and pituitary. Liang Y, Jetton TL, Zimmerman EC, Najafi H, Matschinsky FM, Magnuson MA (1991) J Biol Chem 266(11): 6999-7007
    › Primary publication · 2016311 (PubMed) · Added on 1/16/2014

MeSH Terms

Animals Base Sequence Cell Line Exons Glucokinase Hexokinase Islets of Langerhans Isoenzymes Liver Mice Molecular Sequence Data Mutagenesis, Insertional Mutagenesis, Site-Directed Oligonucleotide Probes Pituitary Gland Polymerase Chain Reaction Rats Restriction Mapping RNA, Messenger RNA Splicing Sequence Homology, Nucleic Acid Transfection