Protocol examples copied from the MagLab Protocols collections


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Added on February 6, 2013 at 11:04 AM by Lindner, Jill
Modified on August 22, 2013 at 9:45 AM by Lindner, Jill
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Resources

Resource  Type Keywords ID
Antibiotic selection of gene targeted mESCsProtocoldiphtheria toxin, gene targeting, geneticin, hygromycin B, selectable markers3064
Calculating beta cell massProtocolembedding, fixation, immunohistochemistry, parafin embedding3067
Casting & running agarose gelsProtocolagarose, electrophoresis, gel3059
DNA lysis, extraction and isolation from ES cellsProtocolDNA extraction, DNA Isolation3065
General scheme for cloning in plasmidsProtocolcloning, insert, ligation, vector3060
Gene targeting and exchange vector guidelinesProtocolexchange cassette, gene target3061
Harvesting MEFsProtocolMEFs, mESCs3066
Making a glycerol stock of bacterial DNAProtocolbacteria, glycerol3063
Puregene DNA isolation from earsProtocolDNA extraction, ears, mouse3068
Saphenous vein blood collectionProtocolblood, mouse, saphenous3069
Transformation of E. coliProtocolbacteria, E. coli, transformation3062
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