The following information is intended to provide you, a potential user of the Stocks-Plasmid Resource Collection, with information that improves the description of your plasmid. These guidelines will help you complete your plasmid submission in a consistent format that has annotations that will be helpful to others.
Please prepare your plasmid sequence files in GenBank (.gb) format. GenBank files may be generated in a variety of applications; however, we recommend using Vector NTI if possible. Additional computer based applications that may generate GenBank formatted files include DNA Star, MacVector, and SimVector. Please include as many key features in the GenBank file annotation as possible. Guidelines for sequence file annotation are described below.
Required annotations (if applicable):
If relevant, please include the following additional annotations:
* Restriction enzyme sites should be limited to critical sites. Please do not include every restriction site in the plasmid.
** Primer annotation should be limited to critical regions. Please do not include unnecessary oligonucleotide sequences in your annotation.
Key word usage
The following information is intended to provide you, a potential user of the Stocks-Plasmid Resource Collection, with information that facilitates your plasmid submission. Keywords will help all users to quickly identify which plasmid(s) will best suit his/her research. When entering a plasmid into the Stocks-Plasmids Resource Collection, please follow these guidelines to determine keyword usage.