A basal exchange plasmid containing a flrted (flanked by FRT sites) pgk-hygro selection cassette and the 5.165 kb Rosa 26 gene sequence region deleted by the gene targeting of pRosa26.LCA was cloned into the plasmid pBS.L66.2272 using a recombineering strategy and the Rosa26 BAC DNA clone #58-D17 in EL350 bacteria..

 

Keywords: Rosa26 RMCE PGK HYGRO

Category
Plasmid
Record History
Added on December 23, 2008 at 11:54 AM by Gangula, Rama
Modified on September 5, 2013 at 12:56 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map M9tPL3ZY.png
Genbank File pRosa26.Ex.Hygro.gb
Backbone Vector pBluescript KS+
Construct Size 10,296 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided

Source

Laboratory Mark Magnuson
Made byKathy Shelton
Stock Date December 23, 2008

Inventory Location

Private

Publications / Citations

  1. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA (2011) Dis Model Mech 4(4): 537-47
    › Primary publication · 21324933 (PubMed) · PMC3124063 (PubMed Central) · Added on 11/8/2013

MeSH Terms

Alleles Animals Chromosomes, Artificial, Bacterial Embryo, Mammalian Embryonic Stem Cells Fluorescence Genetic Loci Genetic Vectors Luminescent Proteins Mice Mutagenesis, Insertional Organ Specificity Proteins Rabbits Recombinases RNA, Untranslated