Jud's Basic Antibody staining protocol:  
       
Step Time Temp Reagent
Permeablize 30 minutes RT PBS + 0.2% Triton X 100
Block 1 hour RT PBS-T (0.2% Tween 20) + 0.5% BSA
wash Rinse RT PBS-T 
Primary  Overnight  4C Primary AB in PBS-T (0.2% Tween 20) + 0.5% BSA
Wash 3x 5 min RT PBS-T (0.2% Tween 20)
Secondary 2 hours RT Secondary AB (1:1000) in PBS-T (0.2% Tween 20) + 0.5% BSA
Wash 2x 5 min RT PBS-T (0.2% Tween 20)
Wash 1x 5 min RT PBS
Mount   RT Prolong-gold w/DAPI (10 uL per Adult Pancreatic Section).  Seal with Cover slip

Record History
Added on June 29, 2012 at 12:41 PM by Lindner, Jill
Modified on June 29, 2015 at 8:42 AM by Lindner, Jill
Shared with (contributions)
VCSCBi: Training / Antibodies Master

Antibody Annotations

Clone Name Not Provided
Antigen MafA
Antigen Gene ID Not Provided
Source of Antigen Mouse
Type Polyclonal
Isotype Not Applicable
Group Type Primary
Immunogen Source Peptide
Peptide N-terminal peptide
Raised in Rabbit
Cross Reacts with Unknown
Conjugation Type Not Provided
Antigen Purity Not Provided
Positive Control Not Provided
Concentration Not Provided

Source

Commercial Bethyl Laboratories
Catalog #Not provided
Stock Date Not provided

Inventory Location

Private

Characterization Data

Microscopy

Notes Not provided
Protocols Juds_Basic_Antibody_Staining_P...
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