NOTICE! This is a TEST resource and should not be considered validated or correct in any manner.

These mice were generated using RMCE to insert an exchange vector containing a modified Rosa26 promoter linked to a Cerulean fluorescent protein (CFP) reporter gene into mESCs containing a Loxed Cassette Acceptor (LCA) allele within the Rosa26 gene locus.  The Rosa26 promoter in this mouse was altered by replacing DNA sequences from 228 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box.  This mouse will facilitate studies of retinoic acid signaling in an intact animal. 


Record History
Added on July 19, 2010 at 10:17 AM by Lindner, Jill
Modified on January 9, 2017 at 10:59 AM by Cartailler, Jean-Philippe
Shared with (contributions)
VCSCBi: Training / Mouse Strains Master

Mouse Information

Common Name Rosa26R26-228-DR5-TA-Cerulean
Research Applications Fluorescent proteins
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID Not provided
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleGene Replacement
Targeted GeneName: gene trap ROSA 26, Philippe Soriano
Symbol: Gt(ROSA)26Sor
NCBI: 14910
Allele Name: targeted mutation 1.2
Symbol: Rosa26tm1.2((R26-228-DR5-Cerulean)
Description of Targeting Vector

A Rosa26 RMCE plasmid constructed for use with the ES cell loci targeted by pRosa26.LCA. In this experiment native Rosa26 gene sequence from -228 to +81 was replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean red reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested.


Vector Genbank File pRosa26.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Type of Allele
Exchanged Cassette Gene Name (14910)
Exchanged Cassette Allele Name Rosa26{LCA}
Description of Exchange Vector
Exchanged Cassette Genbank File R26-228-DR5-TA-Cerulean.gb
PCR Genotyping Protocol Not provided
Citations Not provided

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background Not provided
Current Genetic Background 129S6 and C57Bl6/J hybrid
Number of Generations Backcrossed 2 generations backcrossed to C57BL6/J
Strain Description

129S6 germline chimeras were backcrossed for two generations to C57BL/6J.

Attachments

Document r26228dr5tacerh_pcr_protocol.doc - Added on July 27, 2010 at 2:11 PM by Jill Lindner

PCR protocol for genotyping mice


Document R26-228-DR5-TA-Cerulean.png - Added on July 19, 2010 at 10:17 AM by Jill Lindner