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B6;SJL-TgN(ACTFLPe)9205DMY

NOTICE! This is a TEST resource and should not be considered validated or correct in any manner.

This transgenic strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the human ACTB promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wildtype FLP at 37°C and 40°C, respectively. Mice that are hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain may at times complement use of the Cre-loxP system for in vivo genetic engineering.

Record History
Added on June 30, 2010 at 1:20 PM by Lindner, Jill
Modified on January 9, 2017 at 10:59 AM by Cartailler, Jean-Philippe
Shared with (contributions)
VCSCBi: Training / Mouse Strains Master

Mouse Information

Common Name FLPe
Research Applications FLP-FRT system
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID Not provided
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: BAC or Transgene Insertion
Type of Vector Plasmid
Promoter Name: human beta actin
Symbol: Actb
NCBI: 87904
Expressed Gene Name: Flp recombinase
Symbol: FLP1
Description of Transgene A transgenic construct containing a FLP1 recombinase variant controlled by the human ACTB promoter (3 kb flanking sequence, 78-bp 5' untranslated region, 832-bp of exon 1) was injected into fertilized eggs from B6SJLF2 mice.
Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Citations
Publication
High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP. (2000) Nat Genet 25: 139-40 (Added 7/18/2013)
PMID: 10835623

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background B6SJLF2
Current Genetic Background Not provided
Number of Generations Backcrossed >10
Strain Description

Pronuclear micoinjections were performed using fertilized eggs from B6SJLF2 mice. Founder animals were subsequently crossed with C57BL/6J mice. This strain has been backcrossed to C57BL/6J for over ten generations.

Attachments

Document flpe_jax_pcr_protocol.doc - Added on July 27, 2010 at 2:33 PM by Jill Lindner

PCR protocol for genotyping mice

Document flpe_pcr_protocol.doc - Added on July 27, 2010 at 2:44 PM by Jill Lindner

PCR protocol for genotyping mice