NOTICE! This is a TEST resource and should not be considered validated or correct in any manner.

These mice carry a conditional allele for PPARgamma (γ) that can be used in combination with various cre-expressing transgenes.


Record History
Added on July 19, 2010 at 10:17 AM by Lindner, Jill
Modified on January 9, 2017 at 10:59 AM by Cartailler, Jean-Philippe
Shared with (contributions)
VCSCBi: Training / Mouse Strains Master

Mouse Information

Common Name PPARgammalox
Research Applications Cre-lox system
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VUCMR ID Not provided
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleConditional Null
Targeted GeneName: Peroxisome proliferator-activated receptor gamma
Symbol: Pparg
NCBI: 19016
Allele Name: targeted mutation 1.1Mgn
Symbol: Ppargtm1.1Mgn
MGI: 2385456
Description of Targeting Vector Not provided
Vector Genbank File mPPARG.KO.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Citations
Publication
Generation and functional confirmation of a conditional null PPARgamma allele in mice. (2002) Genesis 32: 134-7 (Added 8/13/2010)
PMID: 11857800

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background Not provided
Current Genetic Background Not provided
Number of Generations Backcrossed 12
Strain Description

Mice carrying the Pparglox allele were backcrossed to C57BL/6J for 12 generations.

Attachments

Document Pparg_Jones_et_al_2002_Page_2_Image_0001.jpg - Added on July 19, 2010 at 10:17 AM by Jill Lindner

PPARgamma gene targeting strategy


Document pparglox__wt_pcr_protocol.doc - Added on July 27, 2010 at 12:51 PM by Jill Lindner

PCR protocol for genotyping mice.