The expression vector pGEX-3X was used for eukaryotic expression of a glucokinase (gk) cDNA fragment from pGK.B1.  A rat gk cDNA fragment with an upstream BamHI site and a downstream EcoRI site was generated by linker-primer PCR and ligated into pGEX-3X.  The plasmid is referred to as both pGST.GKB1 and pGEX.GKB1.


Category
Plasmid
Record History
Added on December 23, 2008 at 11:25 AM by Gangula, Rama
Modified on January 6, 2015 at 9:00 AM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map PqQ7J0Fe.png
Genbank File GST.GKB1.gb
Backbone Vector pGEX-3X
Construct Size 6,340 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided

Source

Laboratory Mark Magnuson
Made byKathy Shelton
Stock Date December 23, 2008

Inventory Location

Private

Publications / Citations

  1. Effects of alternate RNA splicing on glucokinase isoform activities in the pancreatic islet, liver, and pituitary. Liang Y, Jetton TL, Zimmerman EC, Najafi H, Matschinsky FM, Magnuson MA (1991) J Biol Chem 266(11): 6999-7007
    › Primary publication · 2016311 (PubMed) · Added on 1/16/2014

MeSH Terms

Animals Base Sequence Cell Line Exons Glucokinase Hexokinase Islets of Langerhans Isoenzymes Liver Mice Molecular Sequence Data Mutagenesis, Insertional Mutagenesis, Site-Directed Oligonucleotide Probes Pituitary Gland Polymerase Chain Reaction Rats Restriction Mapping RNA, Messenger RNA Splicing Sequence Homology, Nucleic Acid Transfection