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B6.129S6-Gt(ROSA)26Sortm1(Pdx1.YFP)Mgn/Vu

This is an unpublished mouse strain.  This mouse line contains bidirectional TetO-regulated genes inserted into the Rosa26LCA allele by RMCE.  In one direction, the TetO/CMV promoter will drive expression of Pdx1.  In the other direction, it will drive the expression of yellow fluorescent protein (YFP).  In this mouse line, when the effector protein rtTA is expressed, Pdx1 and YFP will be over-expressed simultaneously upon administration of doxycyline.

Keywords: YFP TetO Rosa26 Pdx1 Mgn
Record History
Added on June 26, 2012 at 4:18 PM by Magnuson, Mark
Modified on October 31, 2019 at 2:28 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name Rosa26(Pdx1.YFP)Mgn
VCMR ID SY
Date Cryopreserved 2014-08-25
Method of Cryopreservation Sperm
Trial IVF % Fertilization 82.26%

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Allele Name: targeted mutation 1
Symbol: Gt(ROSA)26Sortm1(Pdx1.YFP)Mgn
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol R26.Pdx1.YFP_PCR_genotyping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Strain background:  88.21% C57BL6/J at cryopreservation. 

Homozygous embryonic lethal at E10.5 due to disruption of neighboring Setd5 gene.

Heterozygous viable and fertile.

Attachments

Document Pdx1_YFP_alternative_genotyping_protocol.docx - Added on August 8, 2018 at 10:26 AM by Jennifer Skelton

Document R26.Pdx1.YFP.pptx - Added on February 8, 2018 at 2:52 PM by Jennifer Skelton

Document Rosa26Pdx1YFPhygroPCR_Protocol.docx - Added on December 9, 2016 at 10:29 AM by Hannah Clayton