B6.Cg-Gt(ROSA)26Sor{tm1(Pdx1.YFP)Mgn}/Vu - Mouse Strain Resource - Labnodes

This is an unpublished mouse strain. This mouse line contains bidirectional TetO-regulated genes inserted into the Rosa26^LCAallele by RMCE. In one direction, the TetO/CMV promoter will drive expression of Pdx1. In the other direction, it will drive the expression of yellow fluorescent protein (YFP). In this mouse line, when the effector protein rtTA is expressed, Pdx1 and YFP will be over-expressed simultaneously upon administration of doxycyline.

Keywords: YFP TetO Rosa26 Pdx1 Mgn

Record History: Added on June 26, 2012 at 4:18 PM by Magnuson, Mark
Modified on December 6, 2018 at 9:33 AM by Skelton, Jennifer
Shared with (contributions): VCMR: Vanderbilt Cryopreserved Mouse Repository

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Mouse Information

Common Name	Rosa26^{(Pdx1.YFP)Mgn}
VCMR ID	SY
Date Cryopreserved	2014-08-25
Method of Cryopreservation	Sperm
Trial IVF % Fertilization	82.26%

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Allele	Name: targeted mutation 1 Symbol: Gt(ROSA)26Sor^{tm1(Pdx1.YFP)Mgn}
Zygosity at cryopreservation	Heterozygote
PCR Genotyping Protocol	R26.Pdx1.YFP_PCR_genotyping_protocol.docx
Citations	Not provided

Background Strain Information

Strain Type	Congenic Strain
Chimera/Founder Genetic Background	129S6/SvEvTac
Cryopreservation Strain Background (VCMR)	C57BL/6J
Viability and Fertility Data	Strain background: 88.21% C57BL6/J at cryopreservation. Homozygous embryonic lethal at E10.5 due to disruption of neighboring Setd5 gene. Heterozygous viable and fertile.