This is an unpublished mouse strain.  This mouse line contains bidirectional TetO-regulated genes inserted into the Rosa26LCA allele by RMCE.  In one direction, the TetO/CMV promoter will drive expression of Pdx1.  In the other direction, it will drive the expression of yellow fluorescent protein (YFP).  In this mouse line, when the effector protein rtTA is expressed, Pdx1 and YFP will be over-expressed simultaneously upon administration of doxycyline.

Keywords: YFP TetO Rosa26 Pdx1 Mgn

Record History
Added on June 26, 2012 at 4:18 PM by Magnuson, Mark
Modified on December 6, 2018 at 9:33 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Rosa26(Pdx1.YFP)Mgn
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID SY
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleCassette Acceptor
Targeted GeneName: gene trap ROSA 26, Philippe Soriano
Symbol: Gt(ROSA)26Sor
NCBI: 14910
Allele Name: targeted mutation 1
Symbol: Gt(ROSA)26Sortm1(Pdx1.YFP)Mgn
Description of Targeting Vector

This ES cell line contains a loxed cassette acceptor (LCA) allele in which a 5.17 kb region of the gene has been replaced by a Lox66 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a Lox2272 site.  These features enable use of Recombinase-Mediated Cassette Exchange (RMCE)  for the rapid insertion of various DNAs into the the Rosa26 gene locus. 


Vector Genbank File pRosa26.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol R26.Pdx1.YFP_PCR_genotyping_protocol.docx
Type of Allele Conditional Activating
Exchanged Cassette Gene Name pancreatic and duodenal homeobox 1 (Pdx1)
Exchanged Cassette Allele Name Rosa26{(Pdx1/YFP)Mgn}
Description of Exchange Vector

The phygro66.2272.Pdx1.YFP exchange vector was made on a backbone of a basal  exchange vector which contains Lox66/Lox2272 sites and a flrted (flanked by FRT)  Pgk-Hygro cassette that is used for positive selection of ES cells after RMCE.  Bidirectional Tet-O regulated genes Pdx1 and YFP were inserted between the Pgk-Hygro and Lox2272 sites.

Exchanged Cassette Genbank File phygro66.2272.Pdx1.YFP.gb
PCR Genotyping Protocol Not provided
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 6
Strain Description

Founder chimeras were bred to C57Bl/6J for 6 generations.

88.21% C57Bl6/J at cryopreservation

Cryopreserved in 2014.

Trial IVF 82.26% fertilization rate.

Attachments

Document Pdx1_YFP_alternative_genotyping_protocol.docx - Added on August 8, 2018 at 10:26 AM by Jennifer Skelton


Document R26.Pdx1.YFP.pptx - Added on February 8, 2018 at 2:52 PM by Jennifer Skelton


Document Rosa26Pdx1YFPhygroPCR_Protocol.docx - Added on December 9, 2016 at 10:29 AM by Hannah Clayton