This knock-in line expresses a red fluorescent protein (mCherry) under control of the Nepn gene locus.  The line is unpublished but is shown to exhibit mCherry expression in kidney (see attached PowerPoint images).  

 


Record History
Added on June 23, 2012 at 11:11 AM by Magnuson, Mark
Modified on December 6, 2018 at 9:39 AM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name NepnCherry
VCMR ID QG
Date Cryopreserved 2013-08-12
Method of Cryopreservation Sperm
Trial IVF % Fertilization 86.21%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: targeted mutation 1
Symbol: Nepntm1(Cherry)Mgn
Zygosity at cryopreservation Homozygote
PCR Genotyping Protocol Nepn.Cherry_PCR_genotyping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) CD-1
Viability and Fertility Data

Strain background:  87.5% CD-1 at cryopreservation

Gene targeting was done in 129S6 mESCs.  Upon germline transmission the allele was crossed into a CD1 background.

Homozygous viable.

Heterozygous viable and fertile.

Attachment

Document NephCherry_information.pptx - Added on August 8, 2017 at 12:06 PM by Mark Magnuson

Background information on Nephrocan, design of gene targeting vector, and evidence for expression in kidney