|Research Applications||Not provided|
|MMRRC ID||Not provided|
|Jackson Laboratories Stock No||Not provided|
|Additional Strain Information||Not provided|
|Mutation #1: Targeted Mutagenesis|
|Type of Allele||Global Null|
|Targeted Gene||Name: Nephrocan|
Name: targeted mutation 1
|Description of Targeting Vector||
The targeting vector was made by BAC recombineering. The vector contains a 5' homology arm of 8392 bp and a 3' arm of 3210 bp. The 5' homology arm is followed by an mCherry-rabbit beta globin poly-A sequence and a FRT-flanked PGK-EM7-Neo cassette that is used for positive selection by Neomycin (targeting events). The PGK-EM7-Neo cassette was excised by crossing to a FlpE-expressing mouse.
|Vector Genbank File||
|Allele Map||Not Provided|
|PCR Genotyping Protocol||Nepn.Cherry_PCR_genotyping_protocol.docx|
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Current Genetic Background||CD-1|
|Number of Generations Backcrossed||3|
Gene targeting was done in 129S6 mESCs. Upon germline transmission the allele was crossed into a CD1 background.
87.5% CD-1 at cryopreservation.
Cryopreserved in 2013.
Trial IVF 86.21% fertilization rate.