This knock-in line expresses a red fluorescent protein (mCherry) under control of the Nepn gene locus.  The line is unpublished but is shown to exhibit mCherry expression in kidney (see attached PowerPoint images).  

 


Record History
Added on June 23, 2012 at 11:11 AM by Magnuson, Mark
Modified on December 6, 2018 at 9:39 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name NepnCherry
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID QG
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleGlobal Null
Targeted GeneName: Nephrocan
Symbol: Nepn
NCBI: 66650
Allele Name: targeted mutation 1
Symbol: Nepntm1(Cherry)Mgn
Description of Targeting Vector

The targeting vector was made by BAC recombineering.  The vector contains a 5' homology arm of 8392 bp and a 3' arm of 3210 bp.  The 5' homology arm is followed by an mCherry-rabbit beta globin poly-A sequence and a FRT-flanked PGK-EM7-Neo cassette that is used for positive selection by Neomycin (targeting events).  The PGK-EM7-Neo cassette was excised by crossing to a FlpE-expressing mouse.


Vector Genbank File pbs.dtanepn.cherry.gb
Allele Map Not Provided
PCR Genotyping Protocol Nepn.Cherry_PCR_genotyping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background CD-1
Number of Generations Backcrossed 3
Strain Description

Gene targeting was done in 129S6 mESCs.  Upon germline transmission the allele was crossed into a CD1 background.

87.5% CD-1 at cryopreservation.

Cryopreserved in 2013.

Trial IVF 86.21% fertilization rate.

Attachment

Document NephCherry_information.pptx - Added on August 8, 2017 at 12:06 PM by Mark Magnuson

Background information on Nephrocan, design of gene targeting vector, and evidence for expression in kidney