This is a basic expression vector containing a 4kb Rosa26 promoter element , This vector has Lox66 and Lox2272 sites and can be used for further construction of gene of interest behind Rosa promoter and used for RMCE.

Record History
Added on May 12, 2009 at 3:58 PM by Yuan, Weiping
Modified on September 5, 2013 at 12:40 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map 2f4bKQOJ.png
Genbank File
Backbone Vector pBS
Construct Size 7,105 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided


Laboratory Mark Magnuson
Made bySara Chen
Stock Date May 12, 2009

Inventory Location


Publications / Citations

  1. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA (2011) Dis Model Mech 4(4): 537-47
    › Primary publication · 21324933 (PubMed) · PMC3124063 (PubMed Central) · Added on 11/8/2013

MeSH Terms

Alleles Animals Chromosomes, Artificial, Bacterial Embryo, Mammalian Embryonic Stem Cells Fluorescence Genetic Loci Genetic Vectors Luminescent Proteins Mice Mutagenesis, Insertional Organ Specificity Proteins Rabbits Recombinases RNA, Untranslated