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Note: This plasmid was published with different name pLCA.71/2272

A backbone vector was designed for assembling gene targeting constructs using the recombineering approach and the Cre-lox system.  The vector contains a puromycin-Dthymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo).  These selectable markers are flanked by minimal (34 bp) lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region.


Category
Plasmid
Record History
Added on December 23, 2008 at 11:10 AM by Gangula, Rama
Modified on September 5, 2013 at 12:43 PM by Magnuson, Mark
Shared with (contributions)
Public: Shared Collection

Vector Annotations

Vector Map AFklAKb7.png
Genbank File pLCA.66.2272.gb
Backbone Vector pBluescript KS+
Construct Size 6,666 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene 22733

Source

Laboratory Mark Magnuson
Made byJared Burlison & Kathy Shelton
Stock Date December 23, 2008

Inventory Location

Private

Publications / Citations

  1. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA (2011) Dis Model Mech 4(4): 537-47
    › Primary publication · 21324933 (PubMed) · PMC3124063 (PubMed Central) · Added on 11/8/2013

MeSH Terms

Alleles Animals Chromosomes, Artificial, Bacterial Embryo, Mammalian Embryonic Stem Cells Fluorescence Genetic Loci Genetic Vectors Luminescent Proteins Mice Mutagenesis, Insertional Organ Specificity Proteins Rabbits Recombinases RNA, Untranslated