Mirazul Islam
(Grad Student, Coffey/Lau Labs)

Keywords: VCSCB SPRING seminar

Record History
Added on August 9, 2022 at 12:30 PM by Uttz, Pam
Modified on September 14, 2022 at 3:47 PM by Uttz, Pam
Shared with (contributions)
Public: SPRING Meeting

Meeting Details

Start Date / Time September 21, 2022 at 9:00 AM
End Date / Time September 21, 2022 at 10:00 AM
Duration 1 hour(s)
Location Zoom
Presenter Name Mirazul Islam
Presentation Title “Developmental and temporal recording of whole mouse at single cell resolution”
Status This meeting has already occurred

Meeting Agenda/Notes

Students and Postdocs

CRISPR-Cas9-based technologies have been developed to address a wide range of biological questions, including molecular recording of embryonic development, genetic screening, and tracking tumor evolution at the single-cell resolution. We developed a single-cell lineage tracking platform called Native sgRNA Capture and sequencing (NSC-seq) to leverage CRISPR technology to decipher early lineage branching point of mouse gastrulation. We combined CRISPR-induced mutations with spontaneously accumulating mitochondrial variants to enable highly efficient lineage-tracking of single cells. Applying this platform in a CRISPR-based transgenic mouse line (MARC1) allows us to reconstruct both embryonic and adult developmental lineages at single-cell resolution. In addition, we performed clonal analysis of intestinal tumors in the barcoded ApcMin/+ mouse model and found that there are more than 2 independently arising clones within a tumor, supporting the oligoclonality of intestinal tumor development. Finally, we apply NSC-seq to assess a comprehensive gene expression phenotype for individual gene knockout at the single-cell level using existing whole-genome CRISPR knockout screening plasmid libraries. Overall, NSC-seq enables in vivo developmental and temporal recording and single-cell CRISPR screening at single cell resolution.

Attachment

Document SPRING_Meeting_9.21.22_Islam.pdf - Added on September 14, 2022 at 3:47 PM by Pam Uttz