RMCE was used to replace exon 2 with a loxP site, the full length human SCN5A, an FRT-flanked hygro cassette and a loxP site.  Restriction fragment length polymorphisms were used to confirm that only the human transcripts were expressed in mutant hearts. This KI contains the conserved noncoding sequence 28.

Keywords: Roden Rdn SCN5A

Record History
Added on July 27, 2022 at 11:02 AM by Skelton, Jennifer
Modified on July 27, 2022 at 11:05 AM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name SCN5A Human WT C57
VCMR ID QM
Date Cryopreserved 2013-08-13
Method of Cryopreservation Sperm
Trial IVF % Fertilization 54.00%

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: sodium channel, voltage-gated, type V, alpha; targeted mutation 1, Dan M Roden
Symbol: Scn5atm1(SC5A)Rdn
MGI: 3691483
Zygosity at cryopreservation Heterozygous
PCR Genotyping Protocol Genotyping_Protocol_QM.pdf
Citations
Publication
Recombinase-mediated cassette exchange to rapidly and efficiently generate mice with human cardiac sodium channels. (2006) Genesis 44: 556-64 (Added 2/23/2011)
PMID: 17083109

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129
Cryopreservation Strain Background (VCMR) C57BL/6
Viability and Fertility Data

Viable and fertile