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129S6-Scn5atm4(SCN5A)Rdn/Vu

Wild type human cDNA for voltage gated sodium channel Scn5a containing flag tag.  Wild type human cDNA for Scn5a replaces expression of murine ortholog. 24 base pair flag tag inserted 3540 base pairs 3' of the ATG start site.

Record History
Added on April 6, 2022 at 1:34 PM by Skelton, Jennifer
Modified on April 18, 2022 at 11:31 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Flag Tag SCN5A Human WT 129
VCMR ID QL
Date Cryopreserved 2013-08-13
Method of Cryopreservation Sperm
Trial IVF % Fertilization 33.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: sodium channel, voltage-gated, type V, alpha; targeted mutation 4, Dan M Roden
Symbol: Scn5atm4SCN5A)Rdn
MGI: 5445934
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol Genotyping_Protocol_QL.pdf
Citations
Publication
Striking In vivo phenotype of a disease-associated human SCN5A mutation producing minimal changes in vitro. (2011) Circulation 124: 1001-11 (Added 12/5/2013)
PMID: 21824921

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) 129S6/SvEvTac
Viability and Fertility Data

Viable and fertile