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CRISPR-mediated double-stranded DNA breaks resolve into frameshift mutations more frequently than the designed edit. When a gene or intergenic locus is critical for viability, it can be challenging to obtain live or fertile progeny. We can now reliably inject just one cell of a two-cell embryo to improve survival rates and wild-type tissue contribution, as recently described (PMID:31253768).
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Released July 13, 2020