In partnership with a VUMC investigator, we developed protocols for genome editing iPSCs using the feeder-free mTeSR1 culture system and commercially-sourced, high-quality genome-editing reagents. We made knockout and point mutation-corrected iPSC clones at high efficiencies without positive or negative selection (see figure, Synthego ICE CRISPR Analysis Tool). If you need a genome-edited iPSC line using either WTC-11 iPSCs or your own patient-derived line, please contact us.

Keywords: gene editing iPSC

Record History
Added on December 14, 2021 at 12:49 PM by Skelton, Jennifer
Modified on December 14, 2021 at 12:51 PM by Skelton, Jennifer
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