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Most of the projects that VGER performs are accomplished by injecting a ribonucleoprotein, consisting of SpCas9 and a guide RNA, along with a single stranded DNA donor into single cell mouse embryos. While this approach works well for DNA deletions and incorporation of DNA sequences under 2 kb, other approaches and strategies can be used to produce genome edited models, including base-editing, prime-editing, double-stranded DNA donors, Cas variants, and alternative CRISPR delivery methods (). We are happy to collaborate with you to implement a custom-tailored genome editing strategy.
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Released July 13, 2020